Use of pten-long leader sequence for transmembrane delivery of molecules
AssigneeThe Trustees of Columbia University in the City of New York
InventorRamon Parsons
Life of this patent
Estimating the term…
Abstract
A composition for delivering cargo molecules across biological membranes is provided comprising (i) a peptide comprising consecutive amino acid residues having the sequence set forth in SEQ ID NO 1 for the transport of a cargo molecule across a biological membrane and (ii) the cargo molecule, wherein the cargo molecule is not a peptide comprising amino acid residues having the sequence set forth in SEQ ID NO 4. Methods of delivering cargo molecules across biological membranes are also provided. Methods of treating a tumor, cancer, a metabolic disorder, and a cardiovascular disorder are also provided.
Claims33
A composition comprising (i) a peptide comprising consecutive amino acid residues 22-173 of the sequence set forth in SEQ ID NO:1, or a portion of amino acid residues 22-173 of SEQ ID NO:1, for the transport of a cargo molecule across a biological membrane and (ii) the cargo molecule, wherein the cargo molecule is not a peptide comprising amino acid residues having the sequence set forth in SEQ ID NO:4.
(canceled)
6-13. (canceled)
15-24. (canceled)
26-29. (canceled)
A method for delivering a cargo molecule into a cell, comprising contacting the cell with a composition comprising (i) a peptide comprising consecutive amino acid residues 22-173 of the sequence set forth in SEQ ID NO:1, or a portion of the amino acid residues set forth in SEQ ID NO:1, for the transport of the cargo molecule across a biological membrane and (ii) the cargo molecule, wherein the cargo molecule is not a peptide comprising amino acid residues having the sequence set forth in SEQ ID NO:4, under conditions permitting the entry of the cargo molecule into the cell.
33-34. (canceled)
37-40. (canceled)
43-55. (canceled)
57-58. (canceled)
A method for treating a tumor in a subject comprising administering to the subject an amount of a composition comprising a peptide comprising amino acid residues 22-173 of SEQ ID NO:1, or a portion of amino acid residues 22-173 of SEQ ID NO:1, conjugated to a cargo molecule, wherein the cargo molecule is not a peptide comprising amino acid residues having the sequence set forth in SEQ ID NO:4 in an amount effective to treat the tumor in the subject.
60-67. (canceled)
A method for treating cancer in a subject comprising administering to the subject an amount of a composition comprising a peptide comprising amino acid residues 22-173 of SEQ ID NO:1, or a portion of amino acid residues 22-173 of SEQ ID NO:1, conjugated to a to a cargo molecule, wherein the cargo molecule is not a peptide comprising amino acid residues having the sequence set forth in SEQ ID NO:4 in an amount effective to treat the cancer in the subject.
70-76. (canceled)
A method for treating a metabolic disorder, wherein the metabolic disorder is characterized by a deficiency in a metabolic enzyme comprising administering to the subject an amount of a composition comprising a peptide comprising amino acid residues 22-173 of SEQ ID NO:1, or portion of amino acid residues 22-173 of SEQ ID NO:1, conjugated to the metabolic enzyme in an amount effective to treat the metabolic disorder in the subject.
78-81. (canceled)
A method for treating diabetes in a subject comprising administering to the subject an amount of a composition comprising a peptide comprising amino acid residues 22-173 of SEQ ID NO:1, or a portion of amino acid residues 22-173 of SEQ ID NO:1, conjugated to a cargo molecule in an amount effective to treat the diabetes in the subject.
83-95. (canceled)
A method for treating a cardiovascular disease in a subject comprising administering to the subject an amount of a composition comprising a peptide comprising amino acid residues 22-173 of SEQ ID NO:1, or a portion of amino acid residues 22-173 of SEQ ID NO:1, conjugated to a cargo molecule in an amount effective to treat the cardiovascular disease in the subject.
97-114. (canceled)
Dependencies are inferred from the claim text.