BMP-1 Procollagen C-Proteinase for Diagnosis and Treatment of Bone and Soft Tissue Defects and Disorders
AssigneeGenera Istrazivanja d.o.o.
InventorsSlobodan VUKICEVIC, Lovorka GRGUREVIC, Boris MACEK
Life of this patent
Estimating the term…
Abstract
Uses of BMP-1 isoforms for diagnosing and treating defects and disorders of bone and soft tissues are described. Also described is a newly isolated variant of the BMP-1 isoform BMP-1-3.
Claims25
A method of treating ischemic acute renal failure in an individual comprising administering a BMP-1 isoform systemically to the individual after diagnosis of renal injury.
A method of treating ischemia/reperfusion damage to a kidney in an individual comprising:
- administering to the individual one or more antibody molecules specific for one or more BMP-1 isoforms in an amount effective to inhibit ischemia/reperfusion injury in said individual.
A method of treating an individual for a defect or disorder in bone or soft tissue of an individual comprising:
- (a) diagnosing a defect or disorder in a bone or soft tissue in an individual by steps comprising:
- (i) determining the profile of BMP-1 isoforms in the blood and
- (ii) comparing the profile to a standard blood profile of BMP-1 isoforms associated with various defects and disorders,
- (b) administering to the individual an amount of at least one BMP-1 isoform effective to enhance the therapeutic effect of an osteogenic BMP toward the diagnosed defect or disorder, or administering to the individual an amount of one or more antibody molecules specific for one or more BMP-1 isoforms effective to inhibit the effects of said one or more BMP-1 isoforms in the progression of the diagnosed defect or disorder.
A method of pretreating an individual to resolve clots that may occur during thoracic or abdominal surgery comprising administering a BMP-1 isoform to the individual prior to surgery in an amount effective to inhibit the occurrence of clots.
A method of treating acute pancreatitis in an individual comprising administering to the individual a therapeutically effective amount of at least one antibody molecule specific for a BMP-1 isoform.
A method of treating pancreatitis in an individual comprising administering to an individual suffering from pancreatitis, after the acute phase of the inflammatory process, an amount of a BMP-1 isoform in an amount effective to promote pancreatic regeneration.
An osteogenic whole blood-derived coagulum device (WBCD) for treating a bone defect in an individual prepared by the steps comprising:
- (a) preparing a mixture comprising:
- (1) whole blood,
- (2) a BMP-1 isoform,
- (3) an exogenous substance supplying calcium ions, and
- (4) optionally, a mixture of fibrin and thrombin, and
- (5) optionally, an osteogenic BMP;
- (b) incubating the mixture of step (a) until a mechanically stable gel is formed, wherein said mechanically stable gel is a homogenous, cohesive, syringeable, injectable, and malleable coagulum gel.
A kit for preparing an osteogenic whole blood-derived coagulum device (WBCD) comprising:
- (a) a vial containing one or more lyophilized BMP-1 isoform,
- (b) a buffer for reconstituting the lyophilized BMP-1 isoforms(s),
- (c) a syringe for reconstituting the lyophilized BMP-1 isoform(s) in the buffer,
- (d) a vaccutainer for collecting a patient's blood,
- (e) a sterile solution of 1 M calcium chloride,
- (f) a fibrin-thrombin mixture,
- (g) a container for mixing whole blood with the reconstituted BMP-1 isoform(s) and other ingredients,
- (h) a spatula or syringe suitable for applying an osteogenic coagulum to bone ends during open bone repair surgery, and
- (i) instructions for the preparation and use of a WBCD comprised of whole blood mixed with one or more BMP-1 isoforms, calcium chloride and, optionally, a mixture comprising fibrin and thrombin, to form a mechanically stable gel suitable for application to a bone defect, wherein said mechanically stable gel is a homogenous, cohesive, syringeable, injectable, and malleable coagulum gel.
An isolated BMP-1 isoform having the amino acid sequence of SEQ ID NO:4.
An isolated polynucleotide encoding the polypeptide having the amino acid sequence of SEQ ID NO:4.
Dependencies are inferred from the claim text.