Crosslinked polysaccharide beads and their biomedical uses
AssigneesUniversite Paris Diderot—Paris 7, Institut National De La Sante Et De La Recherche Medicale (Inserm)
InventorsDidier Letourneur, Catherine Le Visage, Sidi Mohammed Derkaoui
Life of this patent
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Abstract
The present inventions relates to beads as biocompatible material adapted for use within the human or animal body. Said beads are highly useful for tissue engineering, in situ tissue regeneration, as well as for drug and/or cells delivery. In addition, said beads may support biotechnological applications such as cell carriers.
Claims21
A method for preparing beads comprising the following steps:
- a) preparing an alkaline aqueous solution comprising at least one polysaccharide and a cross linking agent;
- b) dispersing said alkaline aqueous solution into an hydrophobic phase in order to obtain a water-in-oil (w/o) emulsion;
- and
- c) transforming the w/o emulsion into beads by placing said w/o emulsion at a temperature from about 4° C. to about 80° C. for a sufficient time to allow the cross-linking of said at least one polysaccharide;
- wherein,
- said polysaccharide is selected from the group consisting of dextran, pullulan, agar, alginic acid, starch hyaluronic acid, inulin, heparin, fucoidan, chitosan and mixtures thereof;
- and
- step b) is performed in the absence of a substantial amount of surfactant.
A bead obtained by
- a) preparing an alkaline aqueous solution comprising at least one polysaccharide and a cross linking agent;
- b) dispersing said alkaline aqueous solution into an hydrophobic phase in order to obtain a water-in-oil (w/o) emulsion;
- and
- c) transforming the w/o emulsion into beads by placing said w/o emulsion at a temperature from about 4° C. to about 80° C. for a sufficient time to allow the cross-linking of said at least one polysaccharide;
- wherein,
- said polysaccharide is selected from the group consisting of dextran, pullulan, agar, alginic acid, starch hyaluronic acid, inulin, heparin, fucoidan, chitosan and mixtures thereof.
12-17. (canceled)
A method for in situ tissue regeneration of damaged or injured tissue in a subject, comprising the step of administering to the damaged or injured tissue a bead obtained by
- a) preparing an alkaline aqueous solution comprising at least one polysaccharide and a cross linking agent;
- b) dispersing said alkaline aqueous solution into an hydrophobic phase in order to obtain a water-in-oil (w/o) emulsion;
- and
- c) transforming the w/o emulsion into beads by placing said w/o emulsion at a temperature from about 4° C. to about 80° C. for a sufficient time to allow the cross-linking of said at least one polysaccharide;
- wherein,
- said polysaccharide is selected from the group consisting of dextran, pullulan, agar, alginic acid, starch hyaluronic acid, inulin, heparin, fucoidan, chitosan and mixtures thereof.
A method of delivering or carrying a drug, a bioactive substance or a cell to a desired location or tissue within a human or animal, comprising
- i) providing a bead obtained by
- a) preparing an alkaline aqueous solution comprising at least one polysaccharide and a cross linking agent;
- b) dispersing said alkaline aqueous solution into an hydrophobic phase in order to obtain a water-in-oil (w/o) emulsion;
- and
- c) transforming the w/o emulsion into beads by placing said w/o emulsion at a temperature from 4° C. to 80° C. for a sufficient time to allow the cross-linking of said at least one polysaccharide;
- wherein said polysaccharide is selected from the group consisting of dextran, pullulan, agar, alginic acid, starch hyaluronic acid, inulin, heparin, fucoidan, chitosan and mixtures thereof;
- and
- wherein said bead further comprises said drug or said bioactive substance;
- and
- ii) administering said bead to said human or animal so as to deliver said bead to said desired location or tissue.
A method of treating cancer within a human or animal, comprising
- i) providing a bead obtained by
- a) preparing an alkaline aqueous solution comprising at least one polysaccharide and a cross linking agent;
- b) dispersing said alkaline aqueous solution into an hydrophobic phase in order to obtain a water-in-oil (w/o) emulsion;
- and
- c) transforming the w/o emulsion into beads by placing said w/o emulsion at a temperature from 4° C. to 80° C. for a sufficient time to allow the cross-linking of said at least one polysaccharide;
- wherein said polysaccharide is selected from the group consisting of dextran, pullulan, agar, alginic acid, starch hyaluronic acid, inulin, heparin, fucoidan, chitosan and mixtures thereof;
- and
- wherein said bead further comprises said drug or said bioactive substance;
- and
- ii) administering said bead to said human or animal so as to deliver said bead to cancerous tissue within said human or animal body.
Dependencies are inferred from the claim text.