Method for Purifying Antibodies
AssigneeXencor, Inc.
InventorsMatthew J. BERNETT, Gregory L. Moore, John Desjarlais, Rumana Rashid
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Abstract
The invention relates generally to compositions and methods for purifying the desired species from a mixture of desired heterodimer and contaminating homodimer immunoglobulin variants by modifying the isoelectric point(s) of the individual chains.
Claims27
A composition comprising a heterodimer protein comprising:
- a) a first monomer comprising:
- i) a first variant heavy chain constant region;
- ii) a first fusion partner;
- and
- b) a second monomer comprising:
- i) a second variant heavy chain constant region;
- ii) a second fusion partner;
- wherein the pIs of said first and second variant heavy chain constant regions are at least 0.5 logs apart.
A composition according any previous claim wherein one of said variant heavy chain constant regions comprises an amino acid substitution selected from the group consisting of Q196K, P217R, P228R, N276K, H435R and Y436F.
A composition according to 11 further comprising wherein one of said variant heavy chain constant regions further comprises a variant selected from the group consisting of S119E, K133E, K133Q, R133E (in case of IgG2-4), R133Q (in case of IgG2-4), T164E, K205E, K205Q, N208D, K210E, K210Q, K274E, K320E, K322E, K326E, K334E, R355E, K392E, Deletion of K447, adding peptide DEDE at the c-terminus, G137E, N203D, K274Q, R355Q, K392N and Q419E, 349A, 349C, 349E, 349I, 349K, 349S, 349T, 349W, 351E, 351K, 354C, 356K, 357K, 364C, 364D, 364E, 364F, 364G, 364H, 364R, 364T, 364Y, 366D, 366K, 366S, 366W, 366Y, 368A, 368E, 368K, 368S, 370C, 370D, 370E, 370G, 370R, 370S, 370V, 392D, 392E, 394F, 394S, 394W, 394Y, 395T, 395V, 396T, 397E, 397S, 397T, 399K, 401K, 405A, 405S, 407T, 407V, 409D, 409E, 411D, 411E, 411K, 439D, 349C/364E, 349K/351K, 349K/351K/394F, 349K/354C, 349K/394F, 349K/394F/401K, 349K/394Y, 349K/401K, 349K/405A, 349T/351E/411E, 349T/394F, 349T/394F/401K, 349T/394F/411E, 349T/405A, 349T/411E, 351E/364D, 351E/364D/405A, 351E/364E, 351E/366D, 351K/364H/401K, 351K/366K, 364D/370G, 364D/394F, 364E/405A, 364E/405S, 364E/411E, 364E/411E/405A, 364H/394F, 364H/401K, 364H/401K/405A, 364H/405A, 364H/405A/411E, 364Y/370R, 370E/411E, 370R/411K, 395T/397S/405A and 397S/405A.
A composition comprising a heterodimeric antibody comprising:
- a) a first heavy chain comprising a first variant heavy chain constant region;
- and
- b) a second heavy chain comprising a second variant heavy chain constant region;
- wherein the pIs of said first and second variant heavy chain constant regions are at least 0.5 logs apart.
A composition according any previous claim wherein one of said variant heavy chain constant regions comprises an amino acid substitution selected from the group consisting of Q196K, P217R, P228R, N276K, H435R and Y436F.
A composition according to 15 wherein one of said light chains is a variant light chain comprising an amino acid substitution selected from the group consisting of K126E, K126Q, K145E, K145Q, N152D, S156E, K169E, S202E, K207E, an addition of DEDE at the C-terminus, R108Q, Q124E, K126Q, N138D, K145T and Q199E.
A composition according to any of the previous claims wherein said first and said second variant heavy chain constant region comprises CH2 and CH3.
A composition according to any of the previous claims wherein said first and said second variant heavy chain constant region comprises CH1, CH2 and CH3.
A composition according to 20 wherein said first heavy chain comprises 4 amino acid substitutions and said second chain has none.
A method of purifying a heterodimeric protein comprising:
- a) providing a composition according to any of the previous claims;
- b) loading said composition onto an ion exchange column;
- and
- c) collecting the heterodimeric fraction.
A method of treating an individual in need thereof comprising administering the composition of any previous claim.
A composition comprising an antibody variant heavy chain constant region comprising an amino acid substitution selected from the group consisting of Q196K, P217R, P228R, N276K, H435R and Y436F.
Dependencies are inferred from the claim text.