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US 2013/0171059 A1Published application
Dual variable domain immunoglobulins and uses thereof
AssigneeAbbVie Inc.
InventorsTariq GHAYUR, Junjian Liu
Life of this patent
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Abstract
Multivalent and multispecific binding proteins, methods of making, and their uses in the diagnosis, prevention, and/or treatment diseases are provided.
Claims25
A binding protein comprising first and second polypeptide chains, each independently comprising VD1-(X1)n-VD2-C-(X2)n, wherein
- VD1 is a first variable domain;
- VD2 is a second variable domain;
- C is a constant domain;
- X1 is a linker with the proviso that X1 is not CH1;
- X2 is an Fc region;
- and
- n is 0 or 1;
- wherein the VD1 domains on the first and second polypeptide chains form a first functional target binding site and the VD2 domains on the first and second polypeptide chains form a second functional target binding site;
- and
- wherein the binding protein is capable of binding TNF and PGE2, TNF and SOST, TNF and NGF, or TNF and LPA, wherein:
- (i) the variable domains that form a functional target binding site for TNF comprise:
- three CDRs from SEQ ID NO:
- 30 and three CDRs from SEQ ID NO:
- 31,
- three CDRs from SEQ ID NO:
- 32 and three CDRs from SEQ ID NO:
- 33,
- three CDRs from SEQ ID NO:
- 34 and three CDRs from SEQ ID NO:
- 35,
- three CDRs from SEQ ID NO:
- 36 and three CDRs from SEQ ID NO:
- 37,
- three CDRs from SEQ ID NO:
- 38 and three CDRs from SEQ ID NO:
- 39, or
- three CDRs from SEQ ID NO:
- 40 and three CDRs from SEQ ID NO:
- 41;
- and
- (ii) the variable domains that form a functional target binding site for PGE2 comprise:
- three CDRs from SEQ ID NO:
- 46 and three CDRs from SEQ ID NO:
- 47;
- (iii) the variable domains that form a functional target binding site for SOST comprise:
- three CDRs from SEQ ID NO:
- 48 and three CDRs from SEQ ID NO:
- 49;
- (iv) the variable domains that form a functional target binding site for NGF comprise:
- three CDRs from SEQ ID NO:
- 44 and three CDRs from SEQ ID NO:
- 45;
- and
- (vi) the variable domains that form a functional target binding site for LPA comprise:
- three CDRs from SEQ ID NO:
- 42 and three CDRs from SEQ ID NO:
- 43.
44 more elements
A binding protein comprising two first and two second polypeptide chains, each independently comprising VD1-(X1)n-VD2-C-(X2)n, wherein
- VD1 is a first variable domain;
- VD2 is a second variable domain;
- C is a constant domain;
- X1 is a linker with the proviso that X1 is not CH1;
- X2 is an Fc region;
- and
- n is 0 or 1;
- wherein the VD1 domains on each set of first and second polypeptide chains form first functional target binding sites and the VD2 domains on each set of first and second polypeptide chains form a second functional target binding site;
- and
- wherein the binding protein is capable of binding TNF and PGE2, TNF and SOST, TNF and NGF, or TNF and LPA, wherein:
- (i) the variable domains that form a functional target binding site for TNF comprise:
- three CDRs from SEQ ID NO:
- 30 and three CDRs from SEQ ID NO:
- 31,
- three CDRs from SEQ ID NO:
- 32 and three CDRs from SEQ ID NO:
- 33,
- three CDRs from SEQ ID NO:
- 34 and three CDRs from SEQ ID NO:
- 35,
- three CDRs from SEQ ID NO:
- 36 and three CDRs from SEQ ID NO:
- 37,
- three CDRs from SEQ ID NO:
- 38 and three CDRs from SEQ ID NO:
- 39, or
- three CDRs from SEQ ID NO:
- 40 and three CDRs from SEQ ID NO:
- 41;
- (ii) the variable domains that form a functional target binding site for PGE2 comprise:
- three CDRs from SEQ ID NO:
- 46 and three CDRs from SEQ ID NO:
- 47;
- (iii) the variable domains that form a functional target binding site for SOST comprise:
- three CDRs from SEQ ID NO:
- 48 and three CDRs from SEQ ID NO:
- 49;
- (iv) the variable domains that form a functional target binding site for NGF comprise:
- three CDRs from SEQ ID NO:
- 44 and three CDRs from SEQ ID NO:
- 45;
- and
- (vi) the variable domains that form a functional target binding site for LPA comprise:
- three CDRs from SEQ ID NO:
- 42 and three CDRs from SEQ ID NO:
- 43.
43 more elements
A binding protein comprising first and second polypeptide chains, wherein the first polypeptide chain comprises VD1-(X1)n-VD2-C-(X2)n, wherein:
- VD1 is a first heavy chain variable domain;
- VD2 is a second heavy chain variable domain;
- C is a constant domain;
- X1 is a linker with the proviso that X1 is not CH1;
- X2 is an Fc region;
- n is 0 or 1;
- and
- wherein the second polypeptide chain comprises VD1-(X1)n-VD2-C-(X2)n, wherein
- VD1 is a first light chain variable domain;
- VD2 is a second light chain variable domain;
- C is a constant domain;
- X1 is a linker with the proviso that X1 is not CL;
- X2 is an Fc region;
- n is 0 or 1;
- and
- wherein the VD1 domains on the first and second polypeptide chains form a first functional target binding site and the VD2 domains on the first and second polypeptide chains form a second functional target binding site;
- and
- wherein the binding protein is capable of binding TNF and PGE2, TNF and SOST, TNF and NGF, or TNF and LPA, wherein:
- (i) the variable domains that form a functional target binding site for TNF comprise:
- three CDRs from SEQ ID NO:
- 30 and three CDRs from SEQ ID NO:
- 31,
- three CDRs from SEQ ID NO:
- 32 and three CDRs from SEQ ID NO:
- 33,
- three CDRs from SEQ ID NO:
- 34 and three CDRs from SEQ ID NO:
- 35,
- three CDRs from SEQ ID NO:
- 36 and three CDRs from SEQ ID NO:
- 37,
- three CDRs from SEQ ID NO:
- 38 and three CDRs from SEQ ID NO:
- 39, or
- three CDRs from SEQ ID NO:
- 40 and three CDRs from SEQ ID NO:
- 41;
- (ii) the variable domains that form a functional target binding site for PGE2 comprise:
- three CDRs from SEQ ID NO:
- 46 and three CDRs from SEQ ID NO:
- 47;
- (iii) the variable domains that form a functional target binding site for SOST comprise:
- three CDRs from SEQ ID NO:
- 48 and three CDRs from SEQ ID NO:
- 49;
- (iv) the variable domains that form a functional target binding site for NGF comprise:
- three CDRs from SEQ ID NO:
- 44 and three CDRs from SEQ ID NO:
- 45;
- and
- (vi) the variable domains that form a functional target binding site for LPA comprise:
- three CDRs from SEQ ID NO:
- 42 and three CDRs from SEQ ID NO:
- 43.
51 more elements
Dependencies are inferred from the claim text.