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AssigneeGeneral Electric Company
InventorsBing Li, Cathryn Ellen Olsen, David Roger Moore
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A modified porous membrane comprising a polymer coating grafted to a porous membrane is described. The polymer coatings grafted to the porous membranes generally comprise a polymer of variable length of an electron beam (e-beam) reactive moiety, designated “poly-(A)x,” a linkage group that forms a bond between the between the poly-(A)x, and a functional B group available to react with a chemical group on a biomolecule, wherein the polymer coating on the porous membrane facilitates the immobilization of a biomolecule, such as DNA, RNA, a protein, and an antibody, on the porous membrane. The compositions find use in immunoassays, in vitro diagnostic tests, point of care tests, techniques for the isolation of a biomolecule from a biological sample, and other methods that require the immobilization of a biomolecule on a porous membrane. Methods of making these modified porous membranes are also disclosed.
A porous membrane having the structure of Formula (I), wherein Formula (I) is:
A porous membrane comprising a coating of at least one polymer grafted to the porous membrane, wherein the polymer coating on the porous membrane is generated by generating free radicals by any method including e-beam irradiation, ultraviolet irradiation, gamma irradiation, corona discharge, or a chemical initiator, and wherein the polymer coating is permanently bound to the porous membrane.
A solid phase material having the structure of Formula (II), wherein Formula (II) is:
The solid phase material of claim 42, wherein the epoxy group-containing compound is GMA, glycidal acrylate, vinyl glycidyl ether, allyl glycidyl ether, methallyl glycidyl ether.
The solid phase material of claim 43, wherein the epoxy group-containing compound is GMA.
A porous membrane or solid phase material comprising polymers of an epoxy group-containing compound grafted on the porous membrane or the solid phase material.
A device for performing an immunoassay that comprises a modified porous membrane or solid phase material comprising polymers of an epoxy group-containing compound grafted on the porous membrane or the solid phase material.
The device of claim 47, wherein the epoxy group-containing compound is GMA and the porous membrane is a nitrocellulose membrane.
Dependencies are inferred from the claim text.