Cell-based methods and reagents
AssigneeAlnylam Pharmaceuticals, Inc.
InventorsAnthony Rossomando, John M. Maraganore, Stuart Pollard, Muthiah Manoharan
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Abstract
The present invention relates to compositions and methods for isolating cells devoid of unwanted viral contaminants, and to methods for preparing a virus stock substantially devoid of viral contaminants. Virus stocks, cells, and immunogenic reagents produced using such methods are also provided.
Claims56
A method of isolating a cell that is substantially devoid of a target virus, comprising:
- a. contacting a population of cells a portion of which comprises the target virus with an RNA effector molecule that inhibits the growth or replication of the target virus;
- b. detecting the presence of the target virus in each cell;
- and,
- c. isolating at least one cell that is substantially devoid of the target virus.
A method of preparing a virus stock that is substantially devoid of an unwanted target virus, comprising:
- (a) contacting a virus stock that contains unwanted target virus with an agent that binds specifically with the unwanted target virus;
- and
- (b) collecting the unbound virus stock.
A method of preparing a virus stock that is substantially devoid of an unwanted target virus, comprising:
- (a) propagating a virus stock in a host cell in which expression of a receptor for a suspected viral contaminant has been inhibited;
- and
- (b) collecting the virus stock.
A virus stock that is substantially devoid of an unwanted target virus, prepared by the process comprising:
- (a) contacting a virus stock that contains unwanted target virus with an agent that binds specifically with the unwanted target virus;
- and
- (b) collecting the unbound virus stock.
A virus stock that is substantially devoid of an unwanted target virus, prepared by the process comprising:
- (a) propagating a virus stock in a host cell in which expression of a receptor for a suspected viral contaminant has been inhibited;
- and
- (b) collecting the stock virus.
Dependencies are inferred from the claim text.