# US 2013/0160159 A1 · Gemini virus replication inhibitor > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 2013/0160159 A1 - Document ID: US20130160159A1 - Status: Published application - Kind: A1 - Application number: 13/702,473 - Filed: 2011-06-06 - Published: 2013-06-20 - Earliest claimed: 2010-06-07 - Inventors: Takashi Sera - Web page: https://patentgrep.com/patent/US20130160159A1 ## CPC - C07K 14/001: {by chemical synthesis} - A01N 59/16: Heavy metals; Compounds thereof - C07K 14/4702: {Regulators; Modulating activity} - C12N 15/8283: {for virus resistance} - C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00) - A01N: PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF (preservation of food or foodstuff A23); BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES (preparations for medical, dental or toiletry purposes which kill or prevent the growth or proliferation of unwanted organisms A61K); PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS - C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00) ## Abstract A replication inhibitor, which is an agent for inhibiting replication of a geminivirus, and comprises a zinc finger protein that can specifically bind to at least full length of stem loop region DNA of the geminivirus, or a part thereof, and can inhibit formation of a stem loop structure. ## Claims ### Claim 1 A replication inhibitor, which comprises a zinc finger protein that can specifically bind to full length DNA of stem loop region of a geminivirus, or one or more partial DNAs selected from the full length DNA, and can inhibit formation of a stem loop structure. ### Claim 2 (depends on 1) The replication inhibitor according to claim 1, which contains a single zinc finger protein that can bind to one partial DNA selected from the full length DNA of the stem region of the geminivirus, or a single zinc finger protein that can bind to two or more partial DNAs selected from the full length DNA. ### Claim 3 (depends on 1) The replication inhibitor according to claim 1, which contains a zinc finger protein formed by binding two or more zinc finger proteins, with a linker or linkers, that can bind to respective two or more partial DNAs selected from the full length DNA of the stem region of the geminivirus. ### Claim 4 (depends on 3) The replication inhibitor according to claim 3, wherein two of the zinc finger proteins consist of a zinc finger protein comprising three zinc finger domains and a zinc finger protein comprising six zinc finger domains. ### Claim 5 (depends on 3) The replication inhibitor according to claim 3, wherein the linker is a peptide linker. ### Claim 6 (depends on 1) The replication inhibitor according to claim 1, wherein the geminivirus is a virus belonging to the genus Begomovirus. ### Claim 7 (depends on 6) The replication inhibitor according to claim 6, wherein the geminivirus is a tomato yellow leaf curl virus. ### Claim 8 (depends on 1) A nucleic acid encoding the zinc finger protein mentioned in claim 1. ### Claim 9 (depends on 8) A recombinant vector for transformation of a plant, which contains the nucleic acid according to claim 8. ### Claim 10 (depends on 1) An agricultural chemical comprising the zinc finger protein according to claim 1 or a nucleic acid encoding the zinc finger protein as an active ingredient. ### Claim 11 (depends on 1) A method for preventing geminivirus infection of a plant, which comprises the step of applying a prophylactically effective amount of the zinc finger protein according to claim 1 or a nucleic acid encoding the zinc finger protein to the plant. ### Claim 12 A gene recombinant plant, which is a plant having resistance against a geminivirus, and can express the zinc finger protein. ### Claim 13 (depends on 1) A plant transformed by introducing a nucleic acid encoding the zinc finger protein according to claim 1. ### Claim 14 (depends on 1) A method for allowing a plant to acquire resistance against a geminivirus, which comprises the step of transforming the plant by introducing a nucleic acid encoding the zinc finger protein according to claim 1 into the plant. ### Claim 15 (depends on 1) A vector for transformation of a plant, which contains a nucleic acid encoding the zinc finger protein mentioned in claim 1. ### Claim 16 (depends on 4) The replication inhibitor according to claim 4, wherein the linker is a peptide linker. ### Claim 17 (depends on 2) The replication inhibitor according to claim 2, wherein the geminivirus is a virus belonging to the genus Begomovirus. ### Claim 18 (depends on 3) The replication inhibitor according to claim 3, wherein the geminivirus is a virus belonging to the genus Begomovirus. ### Claim 19 (depends on 4) The replication inhibitor according to claim 4, wherein the geminivirus is a virus belonging to the genus Begomovirus. ### Claim 20 (depends on 5) The replication inhibitor according to claim 5, wherein the geminivirus is a virus belonging to the genus Begomovirus.