# US 2013/0158265 A1 · Sitagliptin, salts and polymorphs thereof

> Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt

- Number: US 2013/0158265 A1
- Document ID: US20130158265A1
- Status: Published application
- Kind: A1
- Application number: 13/819,252
- Filed: 2011-08-26
- Published: 2013-06-20
- Earliest claimed: 2010-08-27
- Inventors: Dhananjay Govind Sathe; Subhash Vishwanath Damle; Nitin Dnyaneshwar Arote; Rakesh Ramchandra Ambre; Kamlesh Digambar Want; Tushar Anil Naik
- Web page: https://patentgrep.com/patent/US20130158265A1

## CPC

- C07D 487/04: Ortho-condensed systems
- C07D: HETEROCYCLIC COMPOUNDS (macromolecular compounds C08)

## Abstract

The present invention relates to an improved process for preparation of Sitagliptin or pharmaceutically acceptable salts thereof. The present invention further relates to novel polymorphs of Sitagliptin salts and process for preparation thereof.

## Claims

### Claim 1

A process for preparation of Sitagliptin or pharmaceutically acceptable salts thereof comprising the steps of,
a) hydrogenating methyl 4-(2,4,5-trifluorophenyl)-3-oxobutanoate in presence of less than about 0.5% w/w of (S)—BINAP—RuCl2 with respect to methyl 4-(2,4,5-trifluorophenyl)-3-oxobutanoate to obtain 3(S)-4-(2,4,5-trifluorophenyl)-3-hydroxybutanoic acid; and
b) converting said 3(S)-4-(2,4,5-trifluorophenyl)-3-hydroxybutanoic acid to Sitagliptin or pharmaceutically acceptable salts thereof.

### Claim 2 (depends on 1)

The process as claimed in claim 1, wherein said hydrogenation in step a) is carried out at a temperature of about 60° C. to 80° C. and pressure of about 60 to 80 psi for about 5 to 6 hours in presence of organic acid selected from acetic acid, formic acid, citric acid, lactic acid or tartaric acid.

### Claim 3 (depends on 1)

The process as claimed in claim 1, wherein said conversion in step b) comprises the steps of,
a) treating said 3(S)-4-(2,4,5-trifluorophenyl)-3-hydroxybutanoic acid with protected hydroxylamine in presence of a coupling agent followed by cyclocondensation in presence of phosphine ligand and azodicarboxylate to obtain N-Benzyloxy-4(R)[1-methyl-(2,4,5-trifluorophenyl)]-2-oxoazetidine;
b) subjecting said N-Benzyloxy-4(R)-[1-methyl-(2,4,5-trifluorophenyl)]-2-oxoazetidine to ring opening followed by treatment with 3-(trifluoromethyl)-5H,6H,7H, 8H-[1,2,4]triazolo[4,3-a]pyrazine or its salt in presence of a coupling agent to obtain 3(R)-3-[(benzyloxy)amino]-1-[3-(trifluoromethyl)-5H,6H,7H,8H-[1,2,4]triazolo[4,3-a]pyrazin-7-yl]-4-(2,4,5-trifluorophenyl)butan-1-one;
c) subjecting said 3(R)-3-[(benzyloxy)amino]-1-[3-(trifluoromethyl)-5H,6H,7H, 8H-[1,2,4]triazolo[4,3-a]pyrazin-7-yl]-4-(2,4,5-trifluorophenyl)butan-1-one to debenzyloxylation in presence of a suitable catalyst and additive to obtain Sitagliptin;
d) optionally converting said Sitagliptin into its pharmaceutically acceptable salts thereof, which is optionally purified.

### Claim 4 (depends on 3)

The process as claimed in claim 3, wherein said coupling agent is selected from N,N′-dicyclohexylcarbodiimide, 1-ethyl-3-(3-dimethyl amino propyl) carbodiimide or N,N′-diisopropylcarbodiimide; said phosphine ligand is selected from triphenylphosphine, tri(o-tolyl)phosphine, tributylphosphine or trioctylphosphine; and said azodicarboxylate is selected from diisopropylazodicarboxylate (DIAD), diethylazodicarboxylate (DEAD) or dibenzylazodicarboxylate.

### Claim 5 (depends on 3)

The process as claimed in claim 3, wherein said debenzyloxylation in step c) is carried out using hydrogen gas at a temperature of about 30 to 50° C. and pressure of about 30 to 50 psi for about 3 to 6 hours; said suitable catalyst is selected from palladium, platinum, rhodium or nickel on supports such as carbon, silica or alumina, oxides thereof or salts thereof; and said additive is selected from benzyl chloride, benzyl bromide, benzyl iodide or substituted derivatives thereof.

### Claim 6

A process for preparation of Sitagliptin or pharmaceutically acceptable salts thereof comprising the steps of,
a) debenzyloxylation of 3(R)-3-[(benzyloxy)amino]-1-[3-(trifluoromethyl)-5H, 6H,7H,8H-[1,2,4]triazolo[4,3-a]pyrazin-7-yl]-4-(2,4,5-trifluoro phenyl)butan-1-one using hydrogen gas in presence of a catalyst and an additive to obtain a reaction mixture, wherein said additive is selected from benzyl chloride, benzyl bromide, benzyl iodide or substituted derivatives thereof;
b) isolating Sitagliptin from said reaction mixture.

### Claim 7 (depends on 6)

The process as claimed in claim 6, wherein said debenzyloxylation is carried out at a temperature of about 40° C. and pressure of about 40 psi for about 4 to 5 hours; and said catalyst is selected from palladium, platinum, rhodium or nickel on supports such as carbon, silica or alumina, oxides thereof or salts thereof.

### Claim 8 (depends on 6)

The process as claimed in claim 6, wherein said debenzyloxylation of 3(R)-3-[(benzyloxy)amino]-1-[3-(trifluoromethyl)-5H,6H,7H,8H-[1,2,4]triazolo[4,3-a]pyrazin-7-yl]-4-(2,4,5-trifluoro phenyl)butan-1-one is carried out in presence of palladium on carbon support and additive selected from benzyl chloride, benzyl bromide, benzyl iodide or substituted derivatives thereof to obtain a reaction mixture.

### Claim 9 (depends on 8)

The process as claimed in claim 8, wherein said reaction mixture is further treated with trithiocyanuric acid to reduce the palladium content prior to isolation of Sitagliptin.

### Claim 10 (depends on 3)

The process as claimed in claim 3, wherein said conversion of Sitagliptin to pharmaceutically acceptable salts thereof comprises the steps of,
a) dissolving Sitagliptin in a suitable solvent selected from methanol, ethanol, n-propanol, isopropanol, butanol, water, acetone, 2-butanone, diethyl ketone, diethyl ether, diisopropyl ether, ethyl acetate, methyl acetate, propyl acetate, butyl acetate or mixture thereof to obtain a solution;
b) adding salt forming agent selected from phosphoric acid, HCl, SOCl2, NH4Cl, HBr, methane sulfonic acid or ethane sulfonic acid to said solution to obtain a mixture;
c) optionally adding an antisolvent to said mixture; and
d) isolating pharmaceutically acceptable salts of Sitagliptin from said mixture.

### Claim 11 (depends on 10)

The process as claimed in claim 10, wherein said salt forming agent is phosphoric acid; said pharmaceutically acceptable salt is Sitagliptin phosphate;
and said suitable solvent is selected from ethanol, isopropanol, water or mixture thereof.

### Claim 12 (depends on 5)

The process as claimed in claim 5 wherein said Sitagliptin or pharmaceutically acceptable salt thereof is substantially free of compound selected from group consisting of 3(R)-3-Amino-1-[3-(trifluoromethyl)-5H, 6H,7H,8H-[1,2,4]triazolo[4,3-a]pyrazin-7-yl]-4-(2,5 -difluorophenyl) butan-1-one; 3(R)-3 -Amino-1-[3 -(trifluoromethyl)-5H,6H,7H,8H-[1,2,4 ]triazolo[4,3 -a]pyrazin-7-yl]-4-(2,4-difluorophenyl)butan-1-one and 3(R)-3 -Amino-1-[3-(trifluoromethyl)-5H, 6H,7H,8H-[1,2,4]triazolo[4,3-a]pyrazin-7-yl]-4-(3,4-difluorophenyl)butan-1-one.

### Claim 13 (depends on 12)

The process as claimed in claim 12 further comprising the use of compound selected from the group consisting of 3(R)-3-Amino-1-[3-(trifluoromethyl)-5H, 6H,7H,8H-[1,2,4]triazolo[4,3-a]pyrazin-7-yl]-4-(2,5-difluorophenyl)butan-1-one; 3(R)-3-Amino-1-[3-(trifluoromethyl)-5H,6H,7H, 8H-[1,2,4]triazolo[4,3-a]pyrazin-7-yl]-4-(2,4-difluorophenyl)butan-1-one and 3(R)-3-Amino-1-[3-(trifluoromethyl)-5H,6H,7H,8H-[1,2,4]triazolo[4,3-a]pyrazin-7-yl]-4-(3,4-difluorophenyl)butan-1-one as a reference marker and/or reference standard in determining the purity of a sample of Sitagliptin or a pharmaceutical dosage form comprising Sitagliptin.

### Claim 14

(canceled)

### Claim 15 (depends on 10)

The process as claimed in claim 10, wherein said salt forming agent is HCl; said pharmaceutically acceptable salt is Sitagliptin HCl; and said suitable solvent is selected from acetone, 2-butanone, diethyl ketone, diethyl ether, isopropanol or mixture thereof.

### Claim 16 (depends on 15)

The process as claimed in claim 15, wherein said Sitagliptin HCl is characterized by X-ray diffraction pattern having peaks at 2-theta values of about 6.50, 7.96, 13.69, 16.01, 17.97, 18.61, 19.72, 20.26, 22.56, 24.63, 25.35, 25.60, 26.98, 29.31 and 31.54 degrees; or X-ray diffraction pattern having peaks at 2-theta values of about 5.18, 10.36, 12.72, 15.59, 16.06, 16.64, 17.27, 17.54, 19.85, 22.54, 23.62, 23.86, 24.22, 25.72, 26.25, 26.94, 28.09, 28.33 and 28.64 degrees; or X-ray diffraction pattern having peaks at 2-theta values of about 7.30, 7.89, 11.80, 15.77, 16.48, 17.86, 18.09, 20.30, 20.51, 20.88, 21.45, 24.05, 24.71, 25.16 and 25.50 degrees.

### Claim 17 (depends on 10)

The process as claimed in claim 10, wherein said salt forming agent is ethane sulfonic acid; said pharmaceutically acceptable salt is Sitagliptin esylate; and said suitable solvent is selected from methanol, ethanol, n-propanol, isopropanol, butanol, ethyl acetate, methyl acetate, propyl acetate, butyl acetate or mixture thereof; and said anti solvent is selected from diisopropyl ether, diethyl ether, methyl tert-butyl ether, THF or 1,4-dioxane.

### Claim 18 (depends on 17)

The process as claimed in claim 17, wherein said Sitagliptin esylate is characterized by X-ray diffraction pattern having peaks at 2-theta values of about 6.83, 10.66, 12.10, 13.30, 13.67, 15.09, 15.65, 17.22, 18.41, 20.55, 21.49, 22.49, 24.36, 25.71, 27.34, 27.84 and 28.34 degrees; or X-ray diffraction pattern having peaks at 2-theta values of about 5.27, 10.63, 14.92, 15.51, 16.85, 19.26, 21.32, 22.48, 23.35, 24.17, 24.36, 25.23, 25.53 and 32.20 degrees; or X-ray diffraction pattern having peaks at 2-theta values of about 6.86, 13.73, 16.47, 20.60, 23.04, 26.89, 27.83, 33.82 and 34.66 degrees.

### Claim 19

(canceled)