# US 2013/0158246 A1 · Optimized promoter sequence

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- Number: US 2013/0158246 A1
- Document ID: US20130158246A1
- Status: Published application
- Kind: A1
- Application number: 13/057,529
- Filed: 2009-08-07
- Published: 2013-06-20
- Earliest claimed: 2008-08-07
- Assignees: Her Majesty the Queen in right of Canada, as represented by the Minister of Health
- Inventors: Gary Kobinger; Heinz Feldmann; Kaylie Tran
- Web page: https://patentgrep.com/patent/US20130158246A1

## CPC

- C07H 21/04: with deoxyribosyl as saccharide radical
- C07K 14/005: from viruses
- C12N 15/86: Viral vectors
- A61K 2039/5256: {expressing foreign proteins}
- C12N 2760/14122: New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
- C12N 2760/14134: Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
- C12N 2800/22: Vectors comprising a coding region that has been codon optimised for expression in a respective host
- C12N 2830/00: Vector systems having a special element relevant for transcription
- C07H: SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS (derivatives of aldonic or saccharic acids C07C, C07D; aldonic acids, saccharic acids C07C59/105, C07C59/285; cyanohydrins C07C255/16; glycals C07D; compounds of unknown constitution C07G; polysaccharides, derivatives thereof C08B; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification C12N15/00; sugar industry C13)
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)

## Abstract

A modified CAG promoter which is capable of driving high levels of expression of sequences of interest inserted downstream therefrom is herein described.

## Claims

### Claim 1

A purified or isolated functional promoter element having at least 70% identity with the nucleotide sequence as set forth in SEQ ID No. 1.

### Claim 2 (depends on 1)

The promoter element according to claim 1 having at least 80% identity with the nucleotide sequence as set forth in SEQ ID No. 1.

### Claim 3 (depends on 1)

The promoter element according to claim 1 having at least 85% identity with the nucleotide sequence as set forth in SEQ ID No. 1.

### Claim 4 (depends on 1)

The promoter element according to claim 1 having at least 90% identity with the nucleotide sequence as set forth in SEQ ID No. 1.

### Claim 5 (depends on 1)

The promoter element according to claim 1 having at least 95% identity with the nucleotide sequence as set forth in SEQ ID No. 1.

### Claim 6 (depends on 1)

The promoter element according to claim 1 having at least 99% identity with the nucleotide sequence as set forth in SEQ ID No. 1.

### Claim 7

A nucleotide sequence encoding a CAG promoter sequence as set forth in SEQ ID No. 1.

### Claim 8

A nucleotide sequence comprising the CAG promoter sequence as set forth in SEQ ID No. 1 operably linked to a nucleic acid sequence comprising a Kozak sequence and a nucleic acid sequence encoding a codon-optimized Ebola virus glycoprotein as set forth in SEQ ID No. 2.