# US 2013/0158237 A1 · Recombinant protein capable of binding specifically and quickly to troponin i derived from human myocardium

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- Number: US 2013/0158237 A1
- Document ID: US20130158237A1
- Status: Published application
- Kind: A1
- Application number: 13/680,925
- Filed: 2012-11-19
- Published: 2013-06-20
- Earliest claimed: 2011-12-16
- Assignees: Panasonic Corporation
- Inventors: Atsushi FUKUNAGA
- Web page: https://patentgrep.com/patent/US20130158237A1

## CPC

- C07K 16/18: against material from animals or humans
- C07K 2317/622: Single chain antibody (scFv)
- C07K 2317/92: Affinity (KD), association rate (Ka), dissociation rate (Kd) or EC50 value
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)

## Abstract

Provided is a recombination protein which binds specifically to troponin I derived from human myocardium. The recombinant protein includes a light chain variable region consisting of the amino acid sequence represented by SEQ ID NO: 63; and a heavy chain variable region consisting of the amino acid sequence represented by SEQ ID NO: 65.

## Claims

### Claim 1

A recombinant protein which binds specifically to troponin I derived from human myocardium, the recombinant protein comprising:
a light chain variable region consisting of the amino acid sequence represented by SEQ ID NO: 63; and
a heavy chain variable region consisting of the amino acid sequence represented by SEQ ID NO: 65.

### Claim 2 (depends on 1)

The recombinant protein according to claim 1, wherein the recombinant protein is an antibody.

### Claim 3 (depends on 1)

The recombinant protein according to claim 1, wherein the recombinant protein is an antibody fragment.

### Claim 4 (depends on 3)

The recombinant protein according to claim 3, wherein the antibody fragment is a Fab antibody fragment.

### Claim 5 (depends on 3)

The recombinant protein according to claim 3, wherein the antibody fragment is a F(ab′)2 antibody fragment.

### Claim 6 (depends on 3)

The recombinant protein according to claim 3, wherein the antibody fragment is an scFv antibody fragment.

### Claim 7

A method for binding a recombinant protein specifically to troponin I derived from human myocardium, the method comprising:
a step (a) of preparing the recombinant protein, wherein
the recombinant protein comprises:
a light chain variable region consisting of the amino acid sequence represented by SEQ ID NO: 63; and
a heavy chain variable region consisting of the amino acid sequence represented by SEQ ID NO: 65; and
a step (b) of bringing the recombinant protein into contact with the troponin I derived from human myocardium to bind the recombinant protein specifically to the troponin I derived from human myocardium.

### Claim 8 (depends on 7)

The method according to claim 7, wherein the recombinant protein is an antibody.

### Claim 9 (depends on 7)

The method according to claim 7, wherein the recombinant protein is an antibody fragment.

### Claim 10 (depends on 9)

The method according to claim 9, wherein the antibody fragment is a Fab antibody fragment.

### Claim 11 (depends on 9)

The method according to claim 9, wherein the antibody fragment is a F(ab′)2 antibody fragment.

### Claim 12 (depends on 9)

The method according to claim 9, wherein the antibody fragment is an scFv antibody fragment.