# US 2013/0158233 A1 · Method for simultaneously detecting an antigen of, and an antibody, against, an infectious microorganism

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- Number: US 2013/0158233 A1
- Document ID: US20130158233A1
- Status: Published application
- Kind: A1
- Application number: 13/435,381
- Filed: 2012-03-30
- Published: 2013-06-20
- Earliest claimed: 2002-05-10
- Assignees: Bio-Rad Innovations
- Inventors: François RIEUNIER; Muriel Feyssaguet; Stéphanie Henriot; Nadine Lambert
- Web page: https://patentgrep.com/patent/US20130158233A1

## CPC

- C07K 14/1833: {Hepatitis C; Hepatitis NANB}
- C07K 14/005: from viruses
- C07K 14/161: {gag-pol, e.g. p55, p24/25, p17/18, p7, p6, p66/68, p51/52, p31/34, p32, p40}
- C07K 16/118: Hepatitis C virus; GB virus C [GBV-C]
- C07K 16/42: against immunoglobulins
- G01N 33/569: for microorganisms, e.g. protozoa, bacteria, viruses
- G01N 33/56983: {Viruses}
- G01N 33/56988: {HIV or HTLV}
- G01N 33/5767: {non-A, non-B hepatitis}
- C07K 2317/32: specific for a neo-epitope on a complex, e.g. antibody-antigen or ligand-receptor
- C12N 2740/16122: New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
- C12N 2740/16222: New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
- C12N 2770/24222: New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
- G01N 2333/16: HIV-1, HIV-2
- G01N 2333/18: Togaviridae; Flaviviridae
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)

## Abstract

The invention relates to a method for detecting, in vitro, an infection with a microorganism, such as the hepatitis C virus, in a biological sample, by simultaneously detecting an antigen of this microorganism and the antibodies against this same antigen, and also to the reagents and kits implementing this method.

## Claims

### Claim at position 1

1-44. (canceled)

### Claim 45

A peptide or polypeptide derived from the capsid protein of HCV, bearing at least one intact epitope site and at least one destroyed epitope site, said destroyed epitope site thus being made incapable of being recognized by an anti-capsid antibody.

### Claim 46 (depends on 45)

The peptide or polypeptide as claimed in claim 45, comprising any one of sequences SEQ ID NO: 4 to SEQ ID NO: 18.

### Claim 47

A peptide or polypeptide derived from the gag protein of an HIV, bearing at least one intact epitope site and at least one destroyed epitope site, said destroyed epitope site thus being made incapable of being recognized by an anti-gag protein antibody.

### Claim 48 (depends on 47)

The peptide or polypeptide as claimed in claim 47, comprising the sequence SEQ ID NO: 22.