# US 2013/0158099 A1 · Methods for Identifying Agents that Inhibit Cell Migration, Promote Cell Adhesion and Prevent Metastasis

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- Number: US 2013/0158099 A1
- Document ID: US20130158099A1
- Status: Published application
- Kind: A1
- Application number: 13/709,685
- Filed: 2012-12-10
- Published: 2013-06-20
- Earliest claimed: 2009-02-20
- Assignees: GenRemedy, LLC
- Inventors: Craig S. Atwood; Sivan Vadakkadath Meethal
- Web page: https://patentgrep.com/patent/US20130158099A1

## CPC

- A61K 31/7088: Compounds having three or more nucleosides or nucleotides
- A61K 31/664: Amides of phosphorus acids
- C07H 21/04: with deoxyribosyl as saccharide radical
- G01N 33/5011: {for testing antineoplastic activity}
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- C07H: SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS (derivatives of aldonic or saccharic acids C07C, C07D; aldonic acids, saccharic acids C07C59/105, C07C59/285; cyanohydrins C07C255/16; glycals C07D; compounds of unknown constitution C07G; polysaccharides, derivatives thereof C08B; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification C12N15/00; sugar industry C13)
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)

## Abstract

Disclosed are methods for identification of agents that modulate cell attachment, cell migration and cell viability. Cancer and primary cells adhered to a matrix are treated with agent(s) that modulate ActRII signaling and cell adhesion. Agents are tested that modulate cell adhesion, detachment, invasion and viability. Agents that modulate the expression, phosphorylation, function and translocation of ActRII signaling pathway members also can predict agents that modulate cell adhesion, detachment, invasion and viability. The methods have utility in identifying agents that prevent cancer cell metastasis, wound dehiscence, aortic dissection and aid retina attachment and skin replacement and fertility.

## Claims

### Claim 1

A composition to induce cell detachment comprising a concentration of an activin receptor II signaling suppressor sufficient to induce cell detachment.

### Claim 2 (depends on 1)

The composition according to claim 1, wherein the activin receptor II signaling suppressor includes activin receptor type IIB antisense having the sequence of SEQ ID NO 3.

### Claim 3 (depends on 1)

The composition according to claim 1, wherein the activin receptor II signaling suppressor includes activin receptor type IIA antisense having the sequence of SEQ ID NO 1.

### Claim 4 (depends on 1)

The composition according to claim 1, further comprising lipofectamine.

### Claim 5 (depends on 1)

The composition according to claim 1, further comprising sense-P oligonucleotide to activin receptor IIB having the sequence of SEQ ID NO 4.

### Claim 6 (depends on 1)

The composition according to claim 1, further comprising antisense-P oligonucleotide to disintegrin and metalloproteinase domain-containing protein 15 having the sequence of SEQ ID NO 5.

### Claim 7 (depends on 1)

The composition according to claim 1, further comprising sense-P oligonucleotide to activin receptor IIB having the sequence of SEQ ID NO 4 and sense-P oligonucleotide to disintegrin and metalloproteinase domain-containing protein 15 having the sequence of SEQ ID NO 6.