# US 2013/0157900 A1 · Structure-activity relationships

> Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt

- Number: US 2013/0157900 A1
- Document ID: US20130157900A1
- Status: Published application
- Kind: A1
- Application number: 13/764,252
- Filed: 2013-02-11
- Published: 2013-06-20
- Earliest claimed: 2007-02-12
- Assignees: Codexis, Inc.
- Inventors: Emily Mundorff; Simon Christopher Davis; Gjalt W. Huisman; Anke Krebber; John H. Grate
- Web page: https://patentgrep.com/patent/US20130157900A1

## CPC

- C12Q 1/25: involving enzymes not classifiable in groups C12Q1/26 {- C12Q1/66}
- C12N 9/00: Enzymes; Proenzymes; Compositions thereof (preparations containing enzymes for cleaning teeth A61K8/66, A61Q11/00; medicinal preparations containing enzymes or proenzymes A61K38/43; enzyme containing detergent compositions C11D; {enzymes with nucleic acid structure, e.g. ribozymes, C12N15/113}); Processes for preparing, activating, inhibiting, separating or purifying enzymes (preparation of malt C12C1/00)
- C12N 15/1093: {General methods of preparing gene libraries, not provided for in other subgroups}
- C12Q 1/00: Measuring or testing processes involving enzymes, nucleic acids or microorganisms (measuring or testing apparatus with condition measuring or sensing means, e.g. colony counters, C12M1/34); Compositions therefor; Processes of preparing such compositions
- C12Q 1/26: involving oxidoreductase
- C40B 10/00: Directed molecular evolution of macromolecules, e.g. RNA, DNA or proteins
- C40B 30/04: by measuring the ability to specifically bind a target molecule, e.g. antibody-antigen binding, receptor-ligand binding
- C40B 40/02: Libraries contained in or displayed by microorganisms, e.g. bacteria or animal cells; Libraries contained in or displayed by vectors, e.g. plasmids; Libraries containing only microorganisms or vectors
- C40B 40/10: Libraries containing peptides or polypeptides, or derivatives thereof
- G01N 33/6803: {General methods of protein analysis not limited to specific proteins or families of proteins}
- G01N 33/6845: {Methods of identifying protein-protein interactions in protein mixtures}
- G16B 20/00: ICT specially adapted for functional genomics or proteomics, e.g. genotype-phenotype associations
- G16B 20/30: Detection of binding sites or motifs
- G16B 20/50: Mutagenesis
- C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- C40B: COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES (in silico combinatorial libraries of nucleic acids, proteins or peptides G16B35/00; in silico combinatorial chemistry G16C20/60)
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)
- G16B: BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY

## Abstract

The present disclosure relates to compositions and methods for screening a plurality of polypeptide variants.

## Claims

### Claim 1

A kit for identifying at least one polypeptide having a desired activity, comprising:
(i) at least one plurality of addressable polypeptides, wherein each member of each plurality reacts with at least one ligand to produce a detectable signal, and wherein at least two members of each plurality are related to a parent polypeptide and react with different ligands; and
(ii) instructions for use.

### Claim 2 (depends on 1)

The kit of claim 1, wherein said at least one ligand is placed on a surface.

### Claim 3 (depends on 2)

The kit of claim 2, wherein said surface is selected from plastic, glass, silica, metal, microparticles, microchips, and beads.

### Claim 4 (depends on 3)

The kit of claim 3, wherein said at least one ligand is immobilized on said surface.

### Claim 5 (depends on 3)

The kit of claim 3, wherein said plastic comprises the wells of a microtiter plate.

### Claim 6 (depends on 5)

The kit of claim 5, wherein the wells of said microtiter plate contain at least one ligand.

### Claim 7 (depends on 6)

The kit of claim 6, wherein the wells of said microtiter plate contain different ligands.

### Claim 8 (depends on 7)

The kit of claim 7, wherein said at least one ligand is immobilized in at least one well of said microtiter plate.

### Claim 9 (depends on 6)

The kit of claim 6, wherein said at least one ligand is in solution in said wells of said microtiter plate.

### Claim 10 (depends on 1)

The kit of claim 1, wherein said at plurality of addressable polypeptides is placed on a surface.

### Claim 11 (depends on 10)

The kit of claim 10, wherein said surface is selected from plastic, glass, silica, metal, microparticles, microchips, and beads.

### Claim 12 (depends on 10)

The kit of claim 10, wherein at least one member of said plurality of addressable polypeptides is immobilized on said surface.

### Claim 13 (depends on 11)

The kit of claim 11, wherein said plastic comprises the wells of a microtiter plate.

### Claim 14 (depends on 13)

The kit of claim 13, wherein the wells of said microtiter plate contain at least one member of said plurality of addressable polypeptides.

### Claim 15 (depends on 6)

The kit of claim 6, wherein the wells of said microtiter plate contain different members of said plurality of addressable polypeptides.

### Claim 16 (depends on 15)

The kit of claim 15, wherein at least one member of said plurality of addressable polypeptides is immobilized in at least one well of said microtiter plate.

### Claim 17 (depends on 14)

The kit of claim 14, wherein said at least one member of said plurality of addressable polypeptide is in solution in said wells of said microtiter plate.

### Claim 18 (depends on 1)

The kit of claim 1, wherein said at least two members of each plurality of addressable polypeptides are related to a parent polypeptide and react with differing levels of activity upon said at least one ligand.

### Claim 19 (depends on 1)

The kit of claim 1, further comprising at least one means of generating a signal.

### Claim 20 (depends on 19)

The kit of claim 19, further comprising at least one means of detecting said signal.