# US 2013/0157370 A1 · Transformation Vector Comprising Transposon, Microorganisms Transformed with the Vector, and Method for Producing L-Lysine Using the Microorganism

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- Number: US 2013/0157370 A1
- Document ID: US20130157370A1
- Status: Published application
- Kind: A1
- Application number: 13/683,096
- Filed: 2012-11-21
- Published: 2013-06-20
- Earliest claimed: 2008-04-10
- Assignees: Cj Cheiljedang Corporation
- Inventors: Jae-Woo JANG; Sang-Jo LIM; Jong-Soo CHOI; Chul-Ha KIM; Jun-Ok MOON
- Web page: https://patentgrep.com/patent/US20130157370A1

## CPC

- C12N 15/77: for Corynebacterium; for Brevibacterium
- C12N 9/22: Ribonucleases {[RNase]; Deoxyribonucleases [DNase]}
- C12N 15/902: {using homologous recombination}
- C12P 13/08: Lysine; Diaminopimelic acid; Threonine; Valine
- C12N 2800/90: Vectors containing a transposable element
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- C12P: FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {(brewing of beer C12C; producing vinegar C12J; producing specific peptides or proteins C07K; producing enzymes C12N9/00; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification C12N15/00; measuring or testing processes involving enzymes or microorganisms C12Q; measuring or testing processes involving nucleic acid amplification reactions C12Q1/6844; fermentation processes to form a food composition, A21 or A23; compounds in general, see the relevant compound class, e.g. C01, C07)}

## Abstract

The present invention relates to a transformation vector comprising the partial fragments of a gene encoding transposase, a microorganism transformed with the vector, and a method of producing lysine using the microorganism.

## Claims

### Claim 1

A method of inactivating a transposase in the chromosome of a Corynebacterium sp. microorganism and expressing a target gene in the Corynebacterium sp. microorganism, the method comprising the steps of:
1) introducing the target gene into a gene encoding the transposase in the chromosome of the Corynebacterium sp. microorganism to transform the Corynebacterium sp. microorganism; and
2) culturing the transformed microorganism.

### Claim 2 (depends on 1)

The method of claim 1, wherein the target gene is introduced using a transformation vector in which the target gene is inserted in between the 5′ terminal and 3′ terminal fragment of a gene encoding a transposase derived from the corynebacterium sp.

### Claim 3 (depends on 2)

The method of claim 2, wherein the target gene is introduced into the gene encoding the transposase.

### Claim 4 (depends on 1)

The method of claim 1, wherein the transposase is an ISCg1 type or ISCg2 type transposase.

### Claim 5 (depends on 1)

The method of claim 1, wherein a nucleic acid sequence encoding the ISCg1 type transposase is represented by SEQ ID NO: 22, and a nucleic acid sequence encoding the ISCg2 type transposase is represented by SEQ ID NO: 29.