# US 2013/0157368 A1 · Induced pluripotent stem cells prepared from human kidney-derived cells

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- Number: US 2013/0157368 A1
- Document ID: US20130157368A1
- Status: Published application
- Kind: A1
- Application number: 13/331,283
- Filed: 2011-12-20
- Published: 2013-06-20
- Earliest claimed: 2011-12-20
- Assignees: Advanced Technologies and Regenerative Medicine, LLC
- Inventors: Charito Buensuceso; Agnieszka Seyda; David C. Colter; Sridevi Dhanaraj; Brian C. Kramer; Jason Elliot Ekert; Amanda Lynn Kauffman
- Web page: https://patentgrep.com/patent/US20130157368A1

## CPC

- C12N 15/86: Viral vectors
- C12N 5/0696: {Artificially induced pluripotent stem cells, e.g. iPS}
- C12N 2501/602: Sox-2
- C12N 2501/603: Oct-3/4
- C12N 2501/604: Klf-4
- C12N 2501/606: c-Myc
- C12N 2506/25: from renal cells, from cells of the urinary tract
- C12N 2510/00: Genetically modified cells
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)

## Abstract

We have disclosed an induced pluripotent stem cell and the method of preparing the induced pluripotent stem cell from a human kidney-derived cell. More particularly, we have disclosed a human kidney-derived iPS cell which may be differentiated into cells of ectoderm, mesoderm, and endoderm lineages.

## Claims

### Claim at position 1

-5. (canceled)

### Claim 6

A method of making a human kidney-derived induced pluripotent cell comprising the steps of:
providing a human kidney-derived cell, wherein the human kidney-derived cell is positive for the expression of HLA-I and CD 44 and at least one of Oct-4, Rex-1, Pax-2, Cadherin-11, FoxD1, WT1, Eya1, HNF3B, CXC-R4, Sox-17, EpoR, BMP2, BMP7, or GDF5; and negative for the expression of CD133 and E-cadherin and at least one of Sox2, FGF4, hTert, Wnt-4, SIX2 or GATA-4, transfecting the human kidney derived-cell with each one of a VSVg murine retrovirus expressing human transcription factor OCT4, a VSVg murine retrovirus expressing human transcription factor SOX2, a VSVg murine retrovirus expressing human transcription factor KLF4, and a VSVg murine retrovirus expressing human transcription factor c-MYC,
culturing the transfected human kidney-derived cell,
identifying an induced pluripotent stem cell,
isolating the induced pluripotent stem cell,
subculturing the induced pluripotent stem cell, and
providing an induced pluripotent stem cell.

### Claim 7 (depends on 6)

The method of claim 6, further comprising transfecting the human kidney-derived cell with a VSVg murine retrovirus expressing human transcription factor p53-shRNA.

### Claim 8

A method of making a human kidney-derived induced pluripotent cell comprising the steps of:
providing a human kidney-derived cell, transfecting the human kidney-derived cells with each one of an mRNA encoding a Oct-4 protein, an mRNA encoding a Sox2 protein, an mRNA encoding a Klf4 protein, an mRNA encoding a c-myc protein, and an mRNA encoding a Lin28 protein,
culturing the transfected human kidney-derived iPS cell,
identifying a induced pluripotent stem cell,
isolating the induced pluripotent stem cell,
subculturing the induced pluripotent stem cell, and
providing an induced pluripotent stem cell.

### Claim 9 (depends on 6)

An induced pluripotent stem cell made by the method of claim 6.

### Claim 10 (depends on 8)

An induced pluripotent stem cell made by the method of claim 8.