# US 2013/0157366 A1 · Rotation System for Cell Growth Chamber of a Cell Expansion System and Method of Use Therefor > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 2013/0157366 A1 - Document ID: US20130157366A1 - Status: Published application - Kind: A1 - Application number: 13/765,857 - Filed: 2013-02-13 - Published: 2013-06-20 - Earliest claimed: 2009-02-18 - Assignees: Terumo BCT, Inc. - Inventors: Paul M. LEUTHAEUSER; Jon A. DODD; Thomas G. DILORENZO - Web page: https://patentgrep.com/patent/US20130157366A1 ## CPC - C12M 23/50: {Means for positioning or orientating the apparatus (C12M41/08 takes precedence)} - B01F 31/00: Mixers with shaking, oscillating, or vibrating mechanisms - B01F 31/20: Mixing the contents of independent containers, e.g. test tubes - C12M 23/42: {Integrated assemblies, e.g. cassettes or cartridges} - C12M 23/48: {Holding appliances; Racks; Supports (holding devices for laboratory apparatus B01L9/00)} - C12M 25/10: {Hollow fibers or tubes (hollow fiber modules in general B01D63/02)} - C12M 25/12: {the culture medium flowing outside the fiber or tube} - C12M 27/10: {Rotating vessel} - C12M 27/14: {Rotation or movement of the cells support, e.g. rotated hollow fibers} - C12M 27/16: {Vibrating; Shaking; Tilting} - C12N 5/0068: {General culture methods using substrates (for specific animal cell type C12N5/06)} - C12M: APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; {APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS} - B01F: MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING (mixing paints B44D3/06) - C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00) ## Abstract A system and method for rotating a cell growth chamber of a cell expansion system includes a rotatable member for engaging a chamber coupling attached to the cell growth chamber. The rotatable member includes an independently operable mechanism for engaging a rotatable fitting associated with the chamber coupling. In at least one embodiment, the chamber coupling is selectively rotatable by turning the rotatable member, thereby rotating the cell growth chamber around a first axis. The cell growth chamber is also selectively rotatable around a second axis by turning the rotatable fitting associated with the chamber coupling. Other novel aspects include a way of attaching the cell growth chamber to the shaft assembly, and a new tube routing clip. ## Claims ### Claim 1 An apparatus for rotating a cell growth chamber of a cell expansion system, the cell growth chamber including a longitudinal axis, the apparatus comprising: means for rolling the cell growth chamber around at least one of: (a) the longitudinal axis of the cell growth chamber; and (b) an axis substantially parallel to the longitudinal axis of the cell growth chamber; and means for pitching the cell growth chamber such that the longitudinal axis of the cell growth chamber rotates. ### Claim 2 (depends on 1) The apparatus of claim 1, wherein the means for rolling comprises a beveled pinion that contacts a sloped surface of a roll collar attached to the cell growth chamber, and wherein when the beveled pinion rotates around a rotational axis of the beveled pinion the roll cotlar rotates the cell growth chamber around the longitudinal axis of the cell growth chamber. ### Claim 3 (depends on 1) The apparatus of claim 1, wherein the means for rolling and the means for pitching comprise independently rotatable coaxial shaft members. ### Claim 4 (depends on 3) The apparatus of claim 3, wherein the means for pitching comprises an outer shaft member, wherein the means for rolling comprises an inner shaft member, and wherein at least a portion of the inner shaft member is located radially to an interior of the outer shaft member to provide the independently rotatable coaxial shaft members. ### Claim 5 (depends on 4) The apparatus of claim 4, further comprising a means for coupling the cell growth chamber to the outer shaft member. ### Claim 6 (depends on 5) The apparatus of claim 5, wherein the means for coupling includes a shaft fitting, the shaft fitting comprising at least one spring member having a beveled distal end and a shoulder, wherein the beveled distal end deflects upon insertion into the outer shaft member, and wherein once the shoulder clears a front edge of a receptacle of the outer shaft member, the at least one spring member moves radially outward and causes the shoulder to engage the front edge of the receptacle to detachably interconnect the cell growth chamber to the outer shaft member. ### Claim 7 (depends on 4) The apparatus of claim 4, wherein the means for rolling comprises a beveled pinion that contacts a sloped surface of a roll collar attached to the cell growth chamber, wherein the beveled pinion is movable in a direction parallel to a shaft rotation axis of the outer shaft member and the inner shaft member. ### Claim 8 (depends on 7) The apparatus of claim 7, wherein the beveled pinion is biased in a longitudinally distal position by a means for biasing. ### Claim 9 (depends on 7) The apparatus of claim 7, further comprising a means for transferring torque located between the inner shaft member and the beveled pinion. ### Claim 10 (depends on 1) The apparatus of claim 1, wherein the means for rolling comprises a beveled pinion that contacts a sloped surface of a roll collar attached to the cell growth chamber, wherein the beveled pinion contacts the sloped surface substantially along a contact line, and wherein the contact line is oriented at an oblique angle relative to the longitudinal axis of the cell growth chamber. ### Claim 11 A method of rotating a cell growth chamber of a cell expansion system around two axes, a first of the two axes being a longitudinal axis of the cell growth chamber, and a second of the two axes being an axis substantially perpendicular to the longitudinal axis of the cell growth chamber, the method comprising: attaching a shaft fitting of a chamber coupling to an outer shaft member of a shaft assembly, wherein the chamber coupling is connected to the cell growth chamber; and causing an inner shaft member located radially to an interior of the outer shaft member to rotate the cell growth chamber around the longitudinal axis of the cell growth chamber. ### Claim 12 (depends on 11) The method of claim 11, further comprising causing the outer shaft member to rotate, thereby rotating the longitudinal axis of the cell growth chamber. ### Claim 13 (depends on 11) The method of claim 11, further comprising: detaching the chamber coupling from the shaft assembly by releasing the shaft fitting; and attaching a second chamber coupling to the shaft assembly.