# US 2013/0157355 A1 · Rockable biocontainer

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- Number: US 2013/0157355 A1
- Document ID: US20130157355A1
- Status: Published application
- Kind: A1
- Application number: 13/331,212
- Filed: 2011-12-20
- Published: 2013-06-20
- Earliest claimed: 2011-12-20
- Assignees: Pall Corporation
- Inventors: Timothy Alan Barrett; Alison Jane West; Kenneth Roy Weight; Adam Peter Westwood; Daniel James Kesselaar
- Web page: https://patentgrep.com/patent/US20130157355A1

## CPC

- C12M 23/46: {Means for fastening}
- C12M 23/14: {Bags}
- C12M 23/48: {Holding appliances; Racks; Supports (holding devices for laboratory apparatus B01L9/00)}
- C12M 27/16: {Vibrating; Shaking; Tilting}
- C12M 41/14: {Incubators; Climatic chambers (per se B01L1/00)}
- C12M 41/48: {Automatic or computerized control (automatic analysis G01N35/00)}
- C12M: APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; {APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS}

## Abstract

Biocontainers, as well as methods and platforms for rocking the biocontainers, are disclosed.

## Claims

### Claim 1

A biocontainer, comprising
a closed container comprising a bottom wall, a top wall, first and second opposing side walls, and third and fourth opposing side walls, the side walls being joined to the top wall and the bottom wall; and at least two ports;
wherein the biocontainer has a substantially rectangular cuboid form, and the biocontainer further comprises at least first and second brackets secured to the container, wherein the first and second brackets are releasably engageable with a platform suitable for supporting the biocontainer.

### Claim 2 (depends on 1)

The biocontainer of claim 1, wherein further comprises at least third and fourth brackets secured to the container, wherein the third and fourth brackets are releasably engageable with a platform suitable for supporting the biocontainer.

### Claim 3 (depends on 1)

The biocontainer of claim 1, wherein the first bracket is secured to the container near a joint between the top wall and the first side wall, and the second bracket is secured to the container near a joint between the top wall and the second opposing side wall.

### Claim 4 (depends on 3)

The biocontainer of claim 3, further comprising a third bracket secured to the container near a joint between the bottom wall and the first side wall, and a fourth bracket secured to the container near a joint between the bottom wall and the second opposing side wall.

### Claim 5 (depends on 1)

The biocontainer of claim 1, further comprising brackets secured to the bottom wall of the container and/or secured to opposing side walls of the container.

### Claim 6 (depends on 2)

The biocontainer of claim 2, wherein the first and third brackets are each secured to the container near or at a joint between the top wall and the first side wall, and near a joint between the bottom wall and the first side wall, and the second and fourth brackets are each secured to the container near a joint between the top wall and the second opposing side wall, and near a joint between the bottom wall and the second opposing side wall.

### Claim 7

A method of processing a cell culture-containing fluid, the method comprising
rocking a biocontainer comprising a substantially cuboid-shaped closed container on a platform along first and second substantially horizontal pivot axes that are perpendicular to one another, wherein the container contains at least a liquid culture medium and cells, and wherein biocontainer further comprises at least first and second brackets secured to the container.

### Claim 8 (depends on 7)

The method of claim 7, wherein the biocontainer further comprises at least third and fourth brackets secured to the container, the brackets being engaged with the platform during rocking.

### Claim 9 (depends on 7)

The method of claim 7, further comprising removing cell-containing liquid samples from the biocontainer.

### Claim 10 (depends on 7)

The method of claim 7, further comprising adjusting pH in the biocontainer.

### Claim 11 (depends on 7)

The method of claim 7, wherein the first horizontal pivot axis has a first rocking angle and the second horizontal pivot axis has a second rocking angle, wherein the first rocking angle differs from the second rocking angle.

### Claim 12 (depends on 7)

The method of claim 7, wherein the first horizontal pivot axis has a first rocking angle and the second horizontal pivot axis has a second rocking angle, wherein the first rocking angle is the same as the second rocking angle.

### Claim 13 (depends on 11)

The method of claim 11, wherein the first rocking angle or the second rocking angle is in the range of from about 1 to about 15 degrees from horizontal.

### Claim 14 (depends on 12)

The method of claim 12, wherein the first rocking angle and the second rocking angle are each in the range of from about 1 to about 15 degrees from horizontal.

### Claim 15 (depends on 7)

The method of claim 7, wherein the cell culture-containing fluid comprises animal cells.

### Claim 16 (depends on 7)

The method of claim 7, wherein the cell culture-containing fluid comprises microbial cells.

### Claim 17 (depends on 7)

The method of claim 7, wherein the cell culture-containing fluid comprises plant cells.

### Claim 18 (depends on 7)

The method of claim 7, further comprising draining at least some of the fluid from the biocontainer, releasing the engagement between the brackets and the platform, and removing the biocontainer from the platform.

### Claim 19

A rockable platform for rocking a biocontainer comprising
a rockable platform suitable for receiving a substantially cuboid-shaped container, the container comprising at least a first bracket and a second bracket secured thereto, wherein the platform includes at least a first bracket engagement portion and a second bracket engagement portion.

### Claim 20 (depends on 19)

The platform of claim 19, wherein the platform includes at least a third bracket engagement portion and a fourth bracket engagement portion, the third bracket engagement portion and the fourth bracket engagement portion being arranged to engage third and fourth brackets secured to the container.

### Claim 21 (depends on 19)

The platform of claim 19, further comprising a heater for heating the biocontainer.

### Claim 22 (depends on 19)

The platform of claim 19, wherein the first bracket engagement portion comprises at least one slot for receiving the first bracket, and the second bracket engagement portion comprises at least one slot for receiving the second bracket.
