# US 2013/0157345 A1 · Stabilization and activation of protease for use at high temperature > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 2013/0157345 A1 - Document ID: US20130157345A1 - Status: Published application - Kind: A1 - Application number: 13/327,810 - Filed: 2011-12-16 - Published: 2013-06-20 - Earliest claimed: 2011-12-16 - Assignees: Ecolab USA Inc. - Inventors: Kim R. Smith; Thomas R. Mohs - Web page: https://patentgrep.com/patent/US20130157345A1 ## CPC - C11D 3/38663: {Stabilised liquid enzyme compositions} - C11D 3/386: Preparations containing enzymes {, e.g. protease or amylase} - D06L 1/16: Multi-step processes - C11D: DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL - D06L: DRY-CLEANING, WASHING OR BLEACHING FIBRES, FILAMENTS, THREADS, YARNS, FABRICS, FEATHERS OR MADE-UP FIBROUS GOODS; BLEACHING LEATHER OR FURS ## Abstract Methods for use of select organic compounds for the activation and stabilization of thermally labile enzymes, including proteases, are disclosed. The invention further relates to compositions of certain organic compounds with enzymes to enable the use of such enzymes at high temperature conditions, such as for warewash temperatures and conditions. In particular, organic activator-stabilizers including heat-deactivated proteases and zwitterionic materials are combined with enzymes according to the invention. As a result, the invention provides enzyme cleaning efficacy for various high temperature conditions, including warewash applications. ## Claims ### Claim 1 A method of cleaning a hard surface comprising: contacting the hard surface with an aqueous use solution composition comprising from about 0.1 ppm. to about 10,000 ppm enzymes, and from about 0.1 ppm to about 10,000 ppm organic activator-stabilizers, wherein said organic activator-stabilizers increase stabilization of said enzymes at temperatures in excess of at least 150° F. up to temperatures of 180° F. ### Claim 2 (depends on 1) The method of claim 1, wherein said enzyme is a protease and said organic activator-stabilizer is a heat deactivated enzyme. ### Claim 3 (depends on 1) The method of claim 1, where said organic activator-stabilizers are selected froth the group consisting of heat-deactivated enzymes, denatured enzymes, zwitterionic materials and combinations of the same, and wherein said zwitterionic materials are members of the group consisting of amphoteric surfactants, amino acids, proteins and combinations of the same. ### Claim 4 (depends on 1) The method of claim 1, wherein said enzyme is selected from the group consisting of protease, amylase, lipase, cellulase, cutinase, gluconase, peroxidase and combinations of the same. ### Claim 5 (depends on 2) The method of claim 2, wherein said protease in said aqueous use solution is between about 1 ppm and about 400 ppm, and wherein said heat deactivated enzyme in said aqueous use solution is between about 1 ppm and 400 ppm. ### Claim 6 (depends on 1) The method of claim 1, wherein said contacting step comprises either adfixing a multi-use solid concentrated composition capable of forming said aqueous use solution to the inside of a cleaning, application (e.g. dishwash or wash apparatus) and contacting a water source, or adding a single use concentrated liquid or solid composition capable of forming said aqueous use solution to a water source to form said aqueous use solution. ### Claim 7 A method of cleaning a hard surface comprising: (a) forming an organic activator-stabilizer; (b) contacting the hard surface with sufficient organic activator-stabilizer to form an aqueous use solution of from about 0.1 ppm to about 10,000 ppm organic activator-stabilizer; and (c) contacting the hard surface with sufficient enzyme to generate from about 0.1 ppm to about 10,000 ppm enzymes in said aqueous use solution, wherein said organic activator-stabilizer increases stabilization of said enzymes at temperatures in excess of at least 150° F. up to temperatures of 180° F. ### Claim 8 (depends on 7) The method of claim 7, wherein said aqueous use solution further comprises a detergent composition. ### Claim 9 (depends on 7) The method of claim 7, wherein said enzyme is a protease and said organic activator-stabilizer is a heat deactivated enzyme. ### Claim 10 (depends on 7) The method of claim 7, wherein said hard surface is selected from the group consisting of textiles, ware, hard surfaces such as health care instruments and combinations of the same. ### Claim 11 (depends on 7) The method of claim 7, wherein the step of forming said organic activator-stabilizer is integrated into a cleaning application. ### Claim 12 (depends on 7) The method of claim 7, wherein said organic activator-stabilizer is a heat deactivated enzyme formed from as a result of adding an enzyme source to a cleaning application involving a water source at a temperature in excess of about 140° F. ### Claim 13 (depends on 7) The method of claim 7, wherein said aqueous use solution comprises between about 1 ppm and about 400 ppm enzyme and between about 1 ppm and 400 ppm organic activator-stabilizer. ### Claim 14 (depends on 8) The method of claim 8, wherein said enzymes and organic activator-stabilizer do not result in any decrease in detergency of said detergent composition. ### Claim 15 An enzyme stabilizing and activating aqueous use solution for use at elevated temperatures comprising: (a) an aqueous use solution; (b) from about 0.1 ppm to about 10,000 ppm enzymes; and (c) from about 0.1 ppm to about 10,000 ppm organic activator-stabilizers selected from the group consisting of heat-deactivated enzymes, denatured enzymes, zwitterionic materials and combinations of the same, and wherein said organic activator-stabilizers increase stabilization of said enzymes at temperatures in excess of at least 150° F. up to temperatures of 180° F. ### Claim 16 (depends on 15) The composition of claim 15, wherein said enzyme is a protease and said organic activator-stabilizer is a heat deactivated enzyme. ### Claim 17 (depends on 15) The compositions of claim 15, wherein said enzyme is selected from the group consisting of protease, amylase, lipase, cellulase, cutinase, gluconase, peroxidase and combinations of the same. ### Claim 18 (depends on 15) The compositions of claim 15, wherein said zwitterionic materials are members of the group consisting of amphoteric surfactants, amino acids, proteins and combinations of the same. ### Claim 19 (depends on 16) The composition of claim 16, wherein the protease in said aqueous use solution is between about 1 ppm and about 400 ppm, and wherein said heat deactivated enzyme in said aqueous use solution is between about 1 ppm and 400 ppm. ### Claim 20 (depends on 15) The composition of claim 15 further comprising a detergent. ### Claim 21 (depends on 9) The method of claim 9, wherein the step of forming said organic activator-stabilizer comprises exposing said enzyme to sufficient heat to deactivate or denature said enzyme to generate said organic activator-stabilizer.