# US 2013/0157340 A1 · Glyphosate resistant 5-enolpyruvylshikimate-3-phosphate (epsp) synthase

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- Number: US 2013/0157340 A1
- Document ID: US20130157340A1
- Status: Published application
- Kind: A1
- Application number: 13/772,007
- Filed: 2013-02-20
- Published: 2013-06-20
- Earliest claimed: 2006-01-23
- Assignees: Board of Trustees of Michigan State University
- Inventors: Donald Penner; Marulak Simarmata
- Web page: https://patentgrep.com/patent/US20130157340A1

## CPC

- C12N 9/1092: {3-Phosphoshikimate 1-carboxyvinyltransferase (2.5.1.19), i.e. 5-enolpyruvylshikimate-3-phosphate synthase}
- A01H 6/463: {Lolium [ryegrass]}
- C07K 14/415: from plants
- C12N 15/8275: {Glyphosate}
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- A01H: NEW PLANTS OR {NON-TRANSGENIC} PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)

## Abstract

A plant comprising SEQ. ID. NO. 2 or a functional portion thereof, wherein SEQ ID NO. 2 is not native to said plant. A glyphosate resistant grass of economic value comprises a nucleic acid molecule that encodes a EPSPS enzyme. In some embodiments, the nucleic acid molecule comprises a sequence of SEQ. ID. NO. 1, or a functional portion thereof. In some embodiments, the EPSPS enzyme can be a polypeptide molecule comprising an amino acid sequence that is essentially of SEQ. ID. NO. 2, or portion thereof. Embodiments include a DNA construct comprising a SEQ. ID. NO. 1 or a functional portion thereof and transgenic methods for inserting the DNA construct into a plant. Some embodiments include non-transgenic glyphosate resistant turfgrasses.

## Claims

### Claim at position 1

1-73. (canceled)

### Claim 74

An isolated glyphosate resistant gene obtained from the germplasm deposited under American Type Cultural Collection as Penner Ryegrass PTA-8157 deposited Jan. 19, 2007.

### Claim 75 (depends on 74)

The isolated glyphosate resistant gene according to claim 74, wherein the gene comprises a DNA sequence at least 93% homologous to SEQ ID NO:1 that encodes a functional glyphosate-resistant 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS).

### Claim 76 (depends on 75)

The isolated glyphosate resistant gene according to claim 75, wherein the gene comprises a DNA sequence 100% identical to SEQ ID NO:1.

### Claim 77

An isolated nucleic acid molecule obtained from the germplasm deposited under American Type Cultural Collection as Penner Ryegrass PTA-8157 deposited Jan. 19, 2007 comprising SEQ ID NO:1 or a functional portion thereof.

### Claim 78 (depends on 77)

The isolated nucleic acid molecule according to claim 77, wherein the nucleic acid molecule encodes a glyphosate-resistant 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) having an amino acid sequence at least 80% homologous to SEQ ID NO:2.

### Claim 79 (depends on 78)

The isolated nucleic acid molecule according to claim 78, wherein the nucleic acid molecule encodes a glyphosate-resistant EPSPS having an amino acid sequence at least 90% homologous to SEQ ID NO:2.

### Claim 80 (depends on 77)

The isolated nucleic acid molecule according to claim 77, wherein the nucleic acid molecule encodes a glyphosate-resistant EPSPS having an amino acid sequence 100% homologous to SEQ ID NO:2.

### Claim 81 (depends on 77)

An isolated DNA construct comprising the isolated nucleic acid molecule according to claim 77.

### Claim 82

A DNA construct comprising a polynucleotide segment that has a base sequence of SEQ ID NO:1, or a functional portion thereof, and that encodes SEQ ID NO:2, the polynucleotide segment being operably attached to expression regulatory elements that can be used by a host cell to express a 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) polypeptide encoded by the polynucleotide segment.

### Claim 83

A recombinant DNA molecule comprising a structural DNA segment that encodes a 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) polypeptide comprising sequence of SEQ ID NO:2, or a functional portion thereof, operably linked to expression regulatory elements that can be used by a host cell to express the EPSPS polypeptide or peptide encoded by the structural DNA segment.

### Claim 84 (depends on 83)

The recombinant DNA molecule according to claim 83, wherein the expression regulatory elements are those that can be used by a plant cell to achieve expression of the structural DNA sequence.

### Claim 85 (depends on 83)

The recombinant DNA molecule according to claim 83, wherein the expression regulatory elements include a plant promoter that is heterologous to the structural DNA sequence.

### Claim 86 (depends on 83)

The recombinant DNA molecule according to claim 83 further comprising a DNA sequence that is operably linked to the structural DNA segment and that encodes an amino-terminal chloroplast transit peptide.

### Claim 87 (depends on 83)

The recombinant DNA molecule according to claim 83, wherein structural DNA segment that encodes the EPSPS polypeptide has a nucleic acid sequence at least 93% identical to SEQ ID NO:1.

### Claim 88

An isolated glyphosate-resistant 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) encoded by a DNA molecule comprising SEQ ID NO:1 or a functional portion thereof.

### Claim 89

An isolated glyphosate resistant 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) comprising the amino acid sequence of SEQ ID NO:2, or a functional portion SEQ ID NO:2, obtained from the germplasm deposited under American Type Cultural Collection as Penner Ryegrass PTA-8157 deposited Jan. 19, 2007.

### Claim 90

An isolated glyphosate resistant protein (GRP) encoded by a DNA molecule obtained from the germplasm deposited under American Type Cultural Collection as Penner Ryegrass PTA-8157 deposited Jan. 19, 2007.