# US 2013/0157336 A1 · Methods and compositions for oxidizing bisphenol a

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- Number: US 2013/0157336 A1
- Document ID: US20130157336A1
- Status: Published application
- Kind: A1
- Application number: 13/517,182
- Filed: 2011-12-19
- Published: 2013-06-20
- Earliest claimed: 2011-12-19
- Assignees: Empire Technology Development LLC
- Inventors: Robert M. Cabrera
- Web page: https://patentgrep.com/patent/US20130157336A1

## CPC

- A62D 3/02: by biological methods, i.e. processes using enzymes or microorganisms
- C08J 7/0427: {with only one layer of a composition containing a polymer binder (with more layers C08J7/042)}
- C08J 7/046: Forming abrasion-resistant coatings; Forming surface-hardening coatings
- C09D 7/63: organic
- C12N 9/00: Enzymes; Proenzymes; Compositions thereof (preparations containing enzymes for cleaning teeth A61K8/66, A61Q11/00; medicinal preparations containing enzymes or proenzymes A61K38/43; enzyme containing detergent compositions C11D; {enzymes with nucleic acid structure, e.g. ribozymes, C12N15/113}); Processes for preparing, activating, inhibiting, separating or purifying enzymes (preparation of malt C12C1/00)
- C12N 9/0061: {Laccase (1.10.3.2)}
- C12N 9/1051: {Hexosyltransferases (2.4.1)}
- C12N 9/13: {transferring sulfur containing groups (2.8)}
- C12N 11/04: entrapped within the carrier, e.g. gel or hollow fibres
- C12N 11/089: obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds
- C12Y 110/03002: Laccase (1.10.3.2)
- C12Y 111/01: Peroxidases (1.11.1)
- C12Y 204/01017: Glucuronosyltransferase (2.4.1.17)
- A62D 2101/20: Organic substances
- A62D 2101/28: containing oxygen, sulfur, selenium or tellurium, i.e. chalcogen
- A62D: CHEMICAL MEANS FOR EXTINGUISHING FIRES OR FOR COMBATING OR PROTECTING AGAINST HARMFUL CHEMICAL AGENTS; CHEMICAL MATERIALS FOR USE IN BREATHING APPARATUS
- C08J: WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H (working, e.g. shaping, of plastics B29)
- C09D: COATING COMPOSITIONS, e.g. PAINTS, VARNISHES OR LACQUERS; FILLING PASTES; CHEMICAL PAINT OR INK REMOVERS; INKS; CORRECTING FLUIDS; WOODSTAINS; PASTES OR SOLIDS FOR COLOURING OR PRINTING; USE OF MATERIALS THEREFOR (cosmetics A61K; processes for applying liquids or other fluent materials to surfaces, in general, B05D; staining wood B27K5/02; glazes or vitreous enamels C03C; natural resins, French polish, drying-oils, driers, turpentine, per se, C09F; polishing compositions other than French polish, ski waxes C09G; adhesives or use of materials as adhesives C09J; materials for sealing or packing joints or covers C09K3/10; materials for stopping leaks C09K3/12; processes for the electrolytic or electrophoretic production of coatings C25D)
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- C12Y: ENZYMES

## Abstract

Disclosed are methods, compositions and kits pertaining to polymer coatings that entrap enzymes, specifically enzymes capable of degrading bisphenol A.

## Claims

### Claim 1

A composition comprising:
a bisphenol A (BPA)-containing substance; and
at least one BPA-degrading layer coated onto the BPA-containing substance, wherein the BPA-degrading layer comprises at least one BPA-degrading enzyme and at least one polymer.

### Claim 2 (depends on 1)

The composition of claim 1, wherein the BPA-containing substance comprises a polycarbonate plastic or an epoxy resin.

### Claim 3 (depends on 1)

The composition of claim 1, wherein the BPA-degrading enzyme is entrapped in the polymer.

### Claim 4 (depends on 1)

The composition of claim 1, wherein the polymer comprises an organic silane.

### Claim 5 (depends on 4)

The composition of claim 4, wherein the organic silane is formed from polymerization of methyltrimethoxysilane and gamma-aminopropyltriethoxysilane.

### Claim 6 (depends on 1)

The composition of claim 1, wherein the BPA-degrading enzyme oxidizes BPA.

### Claim 7 (depends on 1)

The composition of claim 1, wherein the BPA-degrading enzyme is one or more selected from the group consisting of a potato enzyme extract, a laccase, a tyrosinase, or a peroxidase.

### Claim at position 8

8-10. (canceled)

### Claim 11 (depends on 1)

The composition of claim 1, wherein the BPA-degrading enzyme comprises a glucuronosyltransferase.

### Claim at position 10

12-13. (canceled)

### Claim 14 (depends on 1)

The composition of claim 1, wherein the BPA-degrading enzyme comprises a sulfotransferase.

### Claim 15

(canceled)

### Claim 16

A method of treating a bisphenol A (BPA)-containing substance, the method comprising:
applying a BPA-degrading coating to the surface of a BPA-containing substance, wherein the BPA-degrading coating comprises a BPA-degrading enzyme and a polymer.

### Claim 17 (depends on 16)

The method of claim 16, wherein the BPA-degrading coating comprises a BPA-degrading enzyme entrapped in a polymer particle, wherein the polymer particle forms a gel suspended in an adhesive film.

### Claim 18 (depends on 16)

The method of claim 16, wherein the BPA-containing substance is one or more selected from the group consisting of a polycarbonate plastic or an epoxy resin.

### Claim 19 (depends on 16)

The method of claim 16, wherein the BPA-degrading enzyme is entrapped in the polymer.

### Claim 20 (depends on 16)

The method of claim 16, wherein the polymer comprises an organic silane.

### Claim 21 (depends on 20)

The method of claim 20, wherein the organic silane is formed from polymerization of methyltrimethoxysilane and gamma-aminopropyltriethoxysilane.

### Claim 22 (depends on 16)

The method of claim 16, wherein the BPA-degrading enzyme can oxidize BPA.

### Claim 23 (depends on 16)

The method of claim 16, wherein the BPA-degrading enzyme is one or more selected from the group consisting of a potato enzyme extract, a laccase, a tyrosinase, or a peroxidase.

### Claim at position 21

24-26. (canceled)

### Claim 27 (depends on 16)

The method of claim 16, wherein the BPA-degrading enzyme comprises a glucuronosyltransferase.

### Claim at position 23

28-29. (canceled)

### Claim 30 (depends on 16)

The method of claim 16, wherein the BPA-degrading enzyme comprises a sulfotransferase.

### Claim 31

(canceled)

### Claim 32

A method of reducing release of bisphenol A (BPA) from a BPA-containing substance comprising:
providing a BPA-containing substance; and
applying a BPA-degrading coating to the surface of the BPA-containing substance, wherein the BPA-degrading coating comprises at least one BPA-degrading enzyme and a polymer.

### Claim 33 (depends on 32)

The method of claim 32, wherein the coating comprises a BPA-degrading enzyme entrapped in a polymer particle, wherein the polymer particle forms a gel suspended in an adhesive film.

### Claim 34 (depends on 32)

The method of claim 32, wherein the BPA-containing substance comprises a polycarbonate plastic or an epoxy resin.

### Claim 35 (depends on 32)

The method of claim 32, wherein the BPA-degrading enzyme is entrapped in the polymer.

### Claim 36 (depends on 32)

The method of claim 32, wherein the polymer comprises an organic silane.

### Claim 37

(canceled)

### Claim 38 (depends on 32)

The method of claim 32, wherein the BPA-degrading enzyme oxidizes BPA.

### Claim 39 (depends on 32)

The method of claim 32, wherein the BPA-degrading enzyme is one or more selected from the group consisting of a potato enzyme extract, a laccase, a tyrosinase, or a peroxidase.

### Claim at position 34

40-42. (canceled)

### Claim 43 (depends on 32)

The method of claim 32, wherein the BPA-degrading enzyme is a glucuronosyltransferase.

### Claim at position 36

44-45. (canceled)

### Claim 46 (depends on 32)

The method of claim 32, wherein the BPA-degrading enzyme comprises a sulfotransferase.

### Claim 47

(canceled)