# US 2013/0157306 A1 · Method for determining a degree of infection

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- Number: US 2013/0157306 A1
- Document ID: US20130157306A1
- Status: Published application
- Kind: A1
- Application number: 13/819,616
- Filed: 2010-10-18
- Published: 2013-06-20
- Earliest claimed: 2010-10-18
- Assignees: Foss Analytical A/S
- Inventors: Claus Holm
- Web page: https://patentgrep.com/patent/US20130157306A1

## CPC

- C12Q 1/06: Quantitative determination
- G01N 33/5047: {Cells of the immune system}
- G01N 33/5091: {for testing the pathological state of an organism}
- G01N 33/582: {with fluorescent label}
- G01N 2800/365: Breast disorders, e.g. mastalgia, mastitits, Paget's disease
- C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)

## Abstract

A method for determining a degree of infection comprising the steps of i) preparing an un-isolated sample by adding a differentiating marker, suitably a meta-chromatic stain such as, for example, acridine orange to mammalian milk in an amount sufficient to provide a differentiation between cell types; ii) measuring a differential somatic cell count on the sample by means of a cytometer having a detection system sensitive to differences in the differentiating marker resulting from the marker becoming differently associated with different cell types in the sample; and iii) determining an indication of a degree of infection dependent on the measured differential cell count.

## Claims

### Claim 1

A method for determining a degree of infection comprising the steps of
i) preparing an un-isolated sample by adding a reagent comprising a differentiating marker in an amount sufficient to provide a differentiation between cell types to mammalian milk in a dilution ration of less than 1:200, preferably leas than 1:50 and most preferably less than 1:5;
ii) measuring a cell count for each of one or more types of somatic cell differentiated by the marker on the sample by means of a flow cytometer having a detection system sensitive to differences in the differentiating marker resulting from the marker becoming differently associated with different cell types in the sample; and
iii) determining an indication of a degree of infection dependent on the measured cell count.

### Claim 2 (depends on 1)

A method as claimed in claim 1 characterised in that determining an indication comprises the step of comparing the measured cell count with one or more reference values each being predetermined to be associated with more degrees of infection.

### Claim 3 (depends on 2)

A method as claimed in claim 2 characterised in that there is an additional step of measuring a total somatic cell count and in that the step of determining an indication further comprises comparing the measured total somatic cell count with one or more reference values each being predetermined to be associated with one or more degrees of infection.

### Claim 4

A method as claimed in any preceding claim characterised in that measuring the cell count comprises identifying and counting a number of polymorphonuclear types of leukocyte.

### Claim 5 (depends on 4)

A method as claimed in claim 4 characterised in that counting the number of leukocytes further comprises identifying and counting one or more of the lymphocyte, monocyte and macrophage type of leukocyte.

### Claim 6 (depends on 5)

A method as claimed in claim 5 characterised in that the counting of the number of leukocytes comprises identifying and counting the number of lymphocytes.

### Claim 7

A method as claimed in any preceding claim characterised in that the mammalian milk is bovine milk and in that determining an indication of a degree of infection comprises determining an indication of one or both the presence and degree of mastitis.

### Claim 8 (depends on 7)

A method as claimed in claim 7 characterised in that the indication of mastitis comprises an indication of sub-clinical mastitis.

### Claim 9 (depends on 7, 8)

A method as claimed in claim 7 or claim 8 characterised in that a degree of infection indicative of a likelihood of developing clinical mastitis is determined.

### Claim 10 (depends on 7)

A method as claimed in claim 7 characterised in that a meta-chromatic stain is employed as the differentiating marker.

### Claim 11 (depends on 10)

A method as claimed in claim 10 characterised in that the meta-chromatic stain is acridine orange in a concentration of greater than 0.2 mg/ml.