# US 2013/0157303 A1 · Sulfated polysaccharide capable of binding to growth factor and use thereof > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 2013/0157303 A1 - Document ID: US20130157303A1 - Status: Published application - Kind: A1 - Application number: 13/819,973 - Filed: 2011-08-31 - Published: 2013-06-20 - Earliest claimed: 2010-09-02 - Assignees: Yasuhiko TABATA; Sumitomo Bakelite Co., Ltd. - Inventors: Yoshiaki Fukunishi; Yasuhiko Tabata - Web page: https://patentgrep.com/patent/US20130157303A1 ## CPC - C08B 37/00: Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof (cellulose D21; {microbiological processes C12P}) - A61K 31/737: Sulfated polysaccharides, e.g. chondroitin sulfate, dermatan sulfate (A61K31/727 takes precedence) - A61P 19/00: Drugs for skeletal disorders - A61P 43/00: Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00 - C08B 37/0003: {General processes for their isolation or fractionation, e.g. purification or extraction from biomass} - C08B 37/006: {Heteroglycans, i.e. polysaccharides having more than one sugar residue in the main chain in either alternating or less regular sequence; Gellans; Succinoglycans; Arabinogalactans; Tragacanth or gum tragacanth or traganth from Astragalus; Gum Karaya from Sterculia urens; Gum Ghatti from Anogeissus latifolia; Derivatives thereof} - C12P 19/04: Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds - G01N 33/74: involving hormones {or other non-cytokine intercellular protein regulatory factors such as growth factors, including receptors to hormones and growth factors} - G01N 2400/40: Glycosaminoglycans, i.e. GAG or mucopolysaccharides, e.g. chondroitin sulfate, dermatan sulfate, hyaluronic acid, heparin, heparan sulfate, and related sulfated polysaccharides - G01N 2800/7023: (Hyper)proliferation - C08B: POLYSACCHARIDES; DERIVATIVES THEREOF (polysaccharides containing less than six saccharide radicals attached to each other by glycosidic linkages C07H; fermentation or enzyme-using processes C12P19/00; sugar industry C13; production of cellulose D21) - A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D) - A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS - C12P: FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {(brewing of beer C12C; producing vinegar C12J; producing specific peptides or proteins C07K; producing enzymes C12N9/00; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification C12N15/00; measuring or testing processes involving enzymes or microorganisms C12Q; measuring or testing processes involving nucleic acid amplification reactions C12Q1/6844; fermentation processes to form a food composition, A21 or A23; compounds in general, see the relevant compound class, e.g. C01, C07)} - G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q) ## Abstract A biological substance which can enhance the proliferation and differentiation of cells; a method for evaluating the proliferation and differentiation of cells, which targets the biological substance; a composition for detecting the proliferation and differentiation of cells, which contains a molecule capable of binding to the biological substance; and a composition for enhancing the proliferation and differentiation of cells, which contains the biological substance. A part of a sulfated polysaccharide secreted from cells binds to a growth factor during the course of the proliferation and differentiation of the cells, that the amount of the sulfated polysaccharide capable of the above-mentioned binding and secreted from the cells correlates strongly with the progression of the proliferation and differentiation of the cells, and that the proliferation and differentiation of cells can be evaluated and the proliferation and differentiation of cells can be enhanced utilizing the sulfated polysaccharide. ## Claims ### Claim 1 A sulfated polysaccharide secreted from cells during the course of cell proliferation and differentiation that binds to growth factor. ### Claim 2 (depends on 1) The sulfated polysaccharide of claim 1, wherein the course of cell proliferation and differentiation is the course of proliferation and differentiation of osteocytes or chondrocytes. ### Claim 3 (depends on 1) The sulfated polysaccharide of claim 1, wherein the growth factor is BMP-2 or bFGF. ### Claim 4 (depends on 1) A method for evaluating cell proliferation and differentiation, the method comprising: detecting the sulfated polysaccharide of claim 1. ### Claim 5 (depends on 1) A composition for detecting cell proliferation and differentiation comprising an active ingredient comprising a molecule that binds to the sulfated polysaccharide of claim 1. ### Claim 6 (depends on 1) A composition for promoting cell proliferation and differentiation comprising an active ingredient comprising the sulfated polysaccharide of claim 1. ### Claim 7 (depends on 1) The sulfated polysaccharide of claim 1, wherein the growth factor is BMP-2. ### Claim 8 (depends on 1) The sulfated polysaccharide of claim 1, wherein the growth factor is bFGF.