# US 2013/0157274 A1 · Magnetic lysis method and device

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- Number: US 2013/0157274 A1
- Document ID: US20130157274A1
- Status: Published application
- Kind: A1
- Application number: 13/765,399
- Filed: 2013-02-12
- Published: 2013-06-20
- Earliest claimed: 2009-09-21
- Assignees: Akonni Biosystems, Inc.; Bio-Rad Laboratories, Inc.
- Inventors: Phillip BELGRADER; Benjamin HINDSON
- Web page: https://patentgrep.com/patent/US20130157274A1

## CPC

- C12N 1/066: by physical processes
- C12M 1/00: Apparatus for enzymology or microbiology
- C12N 1/20: Bacteria; Culture media therefor
- C12N 13/00: Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
- C12Q 1/6806: Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay (C12Q1/6804 takes precedence)
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- C12M: APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; {APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS}
- C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES

## Abstract

A method for lysing cells is disclosed. The method includes stirring cells with a magnetic stir element in the presence of a plurality of cell lysis beads at a speed sufficient to lyse the cells. Also disclosed is a device for lysing cells. The device includes a container having a magnetic stir element and a plurality of cell lysis beads disposed therein. The container is dimensioned to allow rotation of the magnetic stir element inside the container.

## Claims

### Claim at position 1

1-16. (canceled)

### Claim 17

A method for lysing cells within one or more vessels, said method comprising:
placing one or more vessels on a surface along a first axis, wherein each vessel comprises one or more cells, one or more magnetic stirrers, and a plurality of beads; and
rotating a magnet about a second axis in the proximity of said one or more vessels, wherein said second axis passes the center of said magnet, said rotation of said magnet causes said one or more magnetic stirrers in each vessel to tumble.

### Claim 18 (depends on 17)

The method of claim 17, wherein said second axis is parallel to said first axis.

### Claim 19 (depends on 17)

The method of claim 17, wherein said second axis forms an angle with said first axis and wherein said angle is greater than 0 degree and smaller than 180 degrees.

### Claim 20 (depends on 17)

The method of claim 17, wherein said magnet is a permanent magnet.

### Claim 21 (depends on 17)

The method of claim 17, wherein said rotating magnet causes said one or more magnetic stirrers in at least one vessel to rotate at a speed greater than 1000 rpm.

### Claim 22 (depends on 17)

The method of claim 17, wherein said rotating magnet causes said one or more magnetic stirrers in each vessel to rotate for a period of about 1 minute to about 15 minutes.

### Claim 23 (depends on 17)

The method of claim 17, wherein said first axis is vertical to said surface and said rotation of said magnet causes said one or more magnetic stirrers in each vessel to rotate about an axis that is vertical to said surface.

### Claim 24 (depends on 17)

The method of claim 17, wherein said first axis is vertical to said surface and said rotation of said magnet causes said one or more magnetic stirrers in each vessel to rotate about an axis that is parallel to said surface.

### Claim 25 (depends on 17)

The method of claim 17, wherein said second axis is above said one or more vessels.

### Claim 26 (depends on 17)

The method of claim 17, wherein said second axis is beneath said one or more vessels.

### Claim 27 (depends on 17)

The method of claim 17, wherein said second axis is on one side of said one or more vessels.

### Claim 28 (depends on 17)

The method of claim 17, wherein said one or more vessels are in the forms selected from the group consisting of vials, test tubes, wells in a microplate, and flow-chambers.

### Claim 29 (depends on 17)

The method of claim 17, wherein said one or more vessels have a shape that is elongated in a direction parallel to said first axis, and wherein said magnet has a shape that is elongated in a direction parallel to said second axis.

### Claim 30 (depends on 17)

The method of claim 17, wherein said one or more vessels contain two or more types of cells and wherein said rotating step comprises:
rotating said magnet at a first speed that causes lysis of a first type of cells.

### Claim 31 (depends on 30)

The method of claim 30, wherein said rotating step further comprises:
rotating said magnet at a second speed that causes lysis of a second type of cells.

### Claim 32

A device for lysing cells, comprising:
a container comprising a magnetic stir element and a plurality of beads disposed therein, wherein the container is dimensioned to allow rotation of the magnetic stir element inside the container.

### Claim 33 (depends on 32)

The device of claim 32, wherein the beads comprise glass beads, plastic beads, ceramic beads, or a mixture thereof.

### Claim 34 (depends on 32)

The device of claim 32, further comprising:
a magnetic stirrer that produces a rotating magnetic field, wherein the magnetic stir element rotates inside the container when placed within the operation range of the rotating magnetic field.

### Claim 35 (depends on 32)

The device of claim 32, further comprising a container holder configured to hold the container within the rotating magnetic field.

### Claim 36 (depends on 32)

The device of claim 32, further comprising a temperature control module that controls the temperature of said container.

### Claim at position 22

37-40. (canceled)