# US 2013/0156884 A1 · Protease enzymes for increased protein digestion rate and absorption and methods of using the same

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- Number: US 2013/0156884 A1
- Document ID: US20130156884A1
- Status: Published application
- Kind: A1
- Application number: 13/329,861
- Filed: 2011-12-19
- Published: 2013-06-20
- Earliest claimed: 2011-12-19
- Assignees: Triarco Industries, Inc.
- Inventors: Mark L. Anderson
- Web page: https://patentgrep.com/patent/US20130156884A1

## CPC

- A23L 29/06: {Enzymes}
- A23L 33/18: Peptides; Protein hydrolysates
- A23L 33/185: Vegetable proteins
- A23L 33/19: Dairy proteins
- A23L 33/195: Proteins from microorganisms
- A61K 35/20: Milk; Whey; Colostrum
- A61K 36/48: Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
- A61K 38/168: {from plants}
- A61K 38/1709: {from mammals}
- A61K 38/48: acting on peptide bonds (3.4)
- A61K 38/4813: {Exopeptidases (3.4.11. to 3.4.19)}
- A61K 38/482: {Serine endopeptidases (3.4.21)}
- A61K 38/484: {Plasmin (3.4.21.7)}
- A61K 38/488: {Aspartic endopeptidases (3.4.23), e.g. pepsin, chymosin, renin, cathepsin E}
- A61K 38/4886: {Metalloendopeptidases (3.4.24), e.g. collagenase}
- C12N 9/62: from Aspergillus
- C12Y 304/11001: Leucyl aminopeptidase (3.4.11.1)
- C12Y 304/21007: Plasmin (3.4.21.7), i.e. fibrinolysin
- C12Y 304/21063: Oryzin (3.4.21.63)
- C12Y 304/23018: Aspergillopepsin I (3.4.23.18)
- C12Y 304/24027: Thermolysin (3.4.24.27)
- A23L: FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF (preservation thereof A23B)
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- C12Y: ENZYMES

## Abstract

A food supplement comprises at least one protease enzyme selected from (i) CAS #9001-92-7, IUB 3.4.23.18 protease from Aspergillus; (ii) CAS #9001-92-7, IUB 3.4.21.7 protease from Bacillus; (iii) CAS #9001-61-0, IUB 3.4.11.1 protease from Aspergillus; (iv) CAS #9074-07-1, IUB 3.4.21.63 protease from Aspergillus; (v) CAS #9073-78-3, IUB 3.4.24.27 protease from Aspergillus; (vi) CAS #9025-49-4, IUB 3.4.23.18 protease from Aspergillus; and (vii) combinations thereof. The food supplement may further comprise a protein, a stabilizer, a matrix modifier, a carrier, a preservative, a bulking agent, a dessicant, an emulsifier, an enzyme coating, or combinations thereof. Disclosed is a method of increasing protein absorption in the gastrointestinal system of a human being comprising the step of ingesting the food supplement as identified above.

## Claims

### Claim 1

A food supplement comprising at least one protease enzyme selected from the group consisting of:
(i) CAS #9001-92-7; IUB 3.4.23.18 protease from Aspergillus:
(ii) CAS #9001-92-7, IUB 3.4.21.7 protease from Bacillus;
(iii) CAS #9001-61-0, IUB 3.4.11.1 protease from Aspergillus:
(iv) CAS #9074-07-1, IUB 3.4.21.63 protease from Aspergillus;
(v) CAS #9073-78-3, IUB 3.4.24.27 protease from Aspergillus;
(vi) CAS #9025-49-4, IUB 3.4.23.18 protease from Aspergillus: and
(vii) combinations thereof.

### Claim 2 (depends on 1)

The food supplement of claim 1, wherein:
CAS #9001-92-7, IUB 3.4.23.18 protease from Aspergillus is from the species oryzae;
CAS #9001-92-7, IUB 3.4.21.7 protease from Bacillus is from the species subtilis;
CAS #9001-61-0, IUB 3.4.11.1 protease from Aspergillus is from the species oryzae;
CAS #9074-07-1, IUB 3.4.21.63 protease from Aspergillus is from the species melleus;
CAS #9073-78-3, IUB 3.4.24.27 protease from Aspergillus is from the species oryzae; and
CAS #9025-49-4, IUB 3.4.23.18 protease from Aspergillus is from the species niger.

### Claim 3 (depends on 1)

The food supplement of claim 1, wherein the at least one protease enzyme is a combination of (i) CAS #9001-92-7, IUB 3.4.23.18 protease from Aspergillus and (iv) CAS #9074-07-1, IUB 3.4.21.63 protease from Aspergillus.

### Claim 4 (depends on 1)

The food supplement of claim 1, wherein the at least one protease enzyme is a combination of (i) CAS #9001-92-7, IUB 3.4.23.18 protease from Aspergillus and (v) CAS #9073-78-3, IUB 3.4.24.27 protease from Aspergillus.

### Claim 5 (depends on 1)

The food supplement of claim 1, wherein the at least one protease enzyme is a combination of (iv) CAS #9074-07-1, IUB 3.4.21.63 protease from Aspergillus and (v) CAS #9073-78-3, IUB 3.4.24.27 protease from Aspergillus.

### Claim 6 (depends on 1)

The food supplement of claim 1, further comprising a protein.

### Claim 7 (depends on 6)

The food supplement of claim 6, wherein the protein is selected from the group consisting of whey, soy, casein, and combinations thereof.

### Claim 8 (depends on 1)

The food supplement of claim 1, further comprising an additional ingredient selected from the group consisting of a stabilizer, a matrix modifier, a carrier, a preservative, a bulking agent, a dessicant, an emulsifier, an enzyme coating, and combinations thereof.

### Claim 9 (depends on 8)

The food supplement of claim 8, wherein the matrix modifier is selected from the group consisting of salts of dihydrogen phosphate, salts of nitric acid, salts of citric acid, salts of polynicotinic acid, salts of picolinic acid, weak organic acids, polyphenolic compounds with at least one pka of 5 or less, and combinations thereof.

### Claim 10 (depends on 6)

The food supplement of claim 6, wherein the at least one protease enzyme is:
(i) CAS #9001-92-7, IUB 3.4.23.18 protease from Aspergillus oryzae;
(ii) CAS #9001-92-7, IUB 3.4.21.7 protease from Bacillus subtilis;
(iii) CAS #9001-61-0, IUB 3.4.11.1 protease from Aspergillus oryzae;
(iv) CAS #9074-07-1, IUB 3.4.21.63 protease from Aspergillus melleus; or
(v) CAS #9073-78-3, IUB 3.4.24.27 protease from Aspergillus oryzae; and
wherein the protein is whey or soy.

### Claim 11 (depends on 3)

The food supplement of claim 3, further comprising a protein selected from the group consisting of whey, soy, casein, and combinations thereof,
wherein CAS #9001-92-7, IUB 3.4.23.18 protease from Aspergillus is from the species oryzae and CAS #9074-07-1, IUB 3.4.21.63 protease from Aspergillus is from the species melleus.

### Claim 12 (depends on 11)

The food supplement of claim 11, further comprising a matrix modifier selected from the group consisting of calcium citrate and chromium (III) polynicotinate, and
wherein the protein is whey or casein.

### Claim 13 (depends on 4)

The food supplement of claim 4, further comprising a protein selected from the group consisting of whey, soy, casein, and combinations thereof,
wherein CAS #9001-92-7, IUB 3.4.23.18 protease from Aspergillus is from the species oryzae and CAS #9073-78-3, IUB 3.4.24.27 protease from Aspergillus is from the species oryzae.

### Claim 14 (depends on 13)

The food supplement of claim 13, wherein the protein is whey.

### Claim 15 (depends on 13)

The food supplement of claim 13, further comprising a matrix modifier selected from the group consisting of calcium citrate and chromium (III) polynicotinate, and
wherein the protein is whey or casein.

### Claim 16 (depends on 5)

The food supplement of claim 5, further comprising a protein selected from the group consisting of whey, soy, casein, and combinations thereof,
wherein CAS #9074-07-1, IUB 3.4.21.63 protease from Aspergillus is from the species melleus and CAS #9073-78-3, IUB 3.4.24.27 protease from Aspergillus is from the species oryzae.

### Claim 17 (depends on 16)

The food supplement of claim 16, wherein the protein is whey.

### Claim 18 (depends on 16)

The food supplement of claim 16, further comprising a matrix modifier selected from the group consisting of calcium citrate and chromium (III) polynicotinate.

### Claim 19 (depends on 1)

A method of increasing protein absorption in the gastrointestinal system of a human being comprising the step of:
ingesting the food supplement of claim 1.

### Claim 20 (depends on 19)

The method of claim 19, wherein protein absorption in the gastrointestinal system is increased in the presence of pepsin.

### Claim 21 (depends on 19)

The method of claim 19, wherein the protein being absorbed is selected from the group consisting of whey, soy, casein, and combinations thereof.

### Claim 22 (depends on 19)

The method of claim 19, wherein the at least one protease enzyme in the food supplement is a combination of (i) CAS #9001-92-7, IUB 3.4.23.18 protease from Aspergillus oryzae and (iv) CAS #9074-07-1, IUB 3.4.21.63 protease from Aspergillus melleus.

### Claim 23 (depends on 19)

The method of claim 19, wherein the at least one protease enzyme in the food supplement is a combination of (i) CAS #9001-92-7, IUB 3.4.23.18 protease from Aspergillus oryzae and (v) CAS #9073-78-3, IUB 3.4.24.27 protease from Aspergillus oryzae.

### Claim 24 (depends on 19)

The method of claim 19, wherein the at least one protease enzyme in the food supplement is a combination of (iv) CAS #9074-07-1, IUB 3.4.21.63 protease from Aspergillus melleus and (v) CAS #9073-78-3, IUB 3.4.24.27 protease from Aspergillus oryzae.

### Claim 25 (depends on 19)

The method of claim 19, further comprising the step of ingesting protein.

### Claim 26 (depends on 25)

The method of claim 25, wherein protein is ingested before, during or after the food supplement is ingested.

### Claim 27 (depends on 25)

The method of claim 25, wherein the food supplement further comprises protein.