# US 2013/0156836 A1 · Chitosan Bornet

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- Number: US 2013/0156836 A1
- Document ID: US20130156836A1
- Status: Published application
- Kind: A1
- Application number: 13/704,840
- Filed: 2011-06-16
- Published: 2013-06-20
- Earliest claimed: 2010-06-18
- Assignees: Kitozyme S.A.
- Inventors: Aurélie Bornet; Sandrine Gautier; Véronique Maquet; Pierre-Louis Teissedre; Daniels Granes; Lucile Pic-Blateyron
- Web page: https://patentgrep.com/patent/US20130156836A1

## CPC

- A01N 43/16: with oxygen as the ring hetero atom
- A01N 25/12: Powders or granules (A01N25/26 takes precedence)
- A23B 2/762: Organic compounds containing nitrogen
- A23B 2/771: Organic compounds containing hetero rings
- A23B 70/10: by addition of preservatives
- A23L 2/52: Adding ingredients (adding preservatives A23B70/10)
- A23L 29/275: of animal origin, e.g. chitin
- A23L 33/10: using additives (addition of substantially indigestible substances A23L33/21)
- A61K 9/1652: {Polysaccharides, e.g. alginate, cellulose derivatives; Cyclodextrin (homeopathic globules A61K9/1623)}
- A61K 9/5036: {Polysaccharides, e.g. gums, alginate; Cyclodextrin}
- A61K 9/5161: {Polysaccharides, e.g. alginate, chitosan, cellulose derivatives; Cyclodextrin}
- B32B 5/16: characterised by features of a layer formed of particles, e.g. chips, powder {or granules (layer formed of natural mineral particles B32B19/00; layer being formed of wood fibres, chips or particles B32B21/02)}
- C08B 37/003: {Chitin, i.e. 2-acetamido-2-deoxy-(beta-1,4)-D-glucan or N-acetyl-beta-1,4-D-glucosamine; Chitosan, i.e. deacetylated product of chitin or (beta-1,4)-D-glucosamine; Derivatives thereof}
- C08L 5/08: Chitin; Chondroitin sulfate; Hyaluronic acid; Derivatives thereof
- C12G 1/02: Preparation of must from grapes; Must treatment and fermentation
- C12H 1/14: with non-precipitating compounds, e.g. sulfiting; Sequestration, e.g. with chelate-producing compounds
- A23V 2002/00: Food compositions, function of food ingredients or processes for food or foodstuffs
- C12G 2200/21: Wine additives, e.g. flavouring or colouring agents
- Y10T 428/2982: Particulate matter [e.g., sphere, flake, etc.]
- A01N: PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF (preservation of food or foodstuff A23); BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES (preparations for medical, dental or toiletry purposes which kill or prevent the growth or proliferation of unwanted organisms A61K); PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A23B: PRESERVATION OF FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES; CHEMICAL RIPENING OF FRUIT OR VEGETABLES
- A23L: FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF (preservation thereof A23B)
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- B32B: LAYERED PRODUCTS, i.e. PRODUCTS BUILT-UP OF STRATA OF FLAT OR NON-FLAT, e.g. CELLULAR OR HONEYCOMB, FORM
- C08B: POLYSACCHARIDES; DERIVATIVES THEREOF (polysaccharides containing less than six saccharide radicals attached to each other by glycosidic linkages C07H; fermentation or enzyme-using processes C12P19/00; sugar industry C13; production of cellulose D21)
- C08L: COMPOSITIONS OF MACROMOLECULAR COMPOUNDS (compositions based on polymerisable monomers C08F, C08G; artificial filaments or fibres D01F; textile treating compositions D06)
- C12G: WINE; PREPARATION THEREOF; ALCOHOLIC BEVERAGES (beer C12C); PREPARATION OF ALCOHOLIC BEVERAGES NOT PROVIDED FOR IN SUBCLASSES C12C OR C12H
- C12H: PASTEURISATION, STERILISATION, PRESERVATION, PURIFICATION, CLARIFICATION OR AGEING OF ALCOHOLIC BEVERAGES; METHODS FOR ALTERING THE ALCOHOL CONTENT OF FERMENTED SOLUTIONS OR ALCOHOLIC BEVERAGES (simulation ageing by flavouring C12G3/06)
- A23V: INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- Y10T: TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION

## Abstract

The invention relates to a chitosan as a micronized powder, to its use for controlling certain yeasts, such as Brettanomyces, and to a method for treating a liquid food. The invention in particular relates to a water-insoluble chitosan as a micronized powder, for which the grain size is comprised between 5 and 50 microns, and to its use in the treatment of liquid foods and/or for controlling Brettanomyces.

## Claims

### Claim at position 1

1-14. (canceled)

### Claim 15

A water-insoluble chitosan in the form of a powder having a grain size comprised between 5 and 50 micrometers (μm).

### Claim 16 (depends on 15)

The chitosan according to claim 15, wherein said chitosan comprises a degree of acetylation (DA) comprised between 0 and 30 mol %.

### Claim 17

A suspension in a liquid of a water-insoluble chitosan, said chitosan being in the form of a powder having a grain size comprised between 5 and 50 micrometers.

### Claim 18 (depends on 17)

The suspension according to claim 17, wherein said liquid is water.

### Claim 19

A method for limiting fungal in a liquid food, wherein said method comprises treating said liquid food with an antifungal agent or a composition comprising said antifungal agent, said antifungal agent being a water-insoluble chitosan in the form of a powder having a grain size comprised between 0.1 and 200 micrometers, said water-insoluble chitosan being at a concentration from 1 to 10 g/hL of said liquid food to be treated.

### Claim 20 (depends on 19)

The method according to claim 19, wherein said chitosan concentration is comprised between 2 and 5 g/hL of said liquid food to be treated.

### Claim 21 (depends on 19)

The method according to claim 19, wherein said grain size is comprised between 5 and 50 micrometers.

### Claim 22 (depends on 19)

The method according to claim 19, wherein said method is for controlling yeasts in a liquid food.

### Claim 23 (depends on 22)

The method according to claim 22, wherein said yeasts are selected from the group consisting of Brettanomyces, Dekkera, B. anomalus, B. bruxellensis, B. claussenii, B. custersianus, B. lambicus, B. naardenensis, B. nanus, and any mixtures thereof.

### Claim 24 (depends on 22)

The method according to claim 22, wherein said grain size is comprised between 5 and 50 micrometers.

### Claim 25

A method for controlling undesirable yeasts in a liquid food of vegetable origin, wherein said method comprises treating said liquid food with an antifungal agent or a composition comprising said antifungal agent, said antifungal agent being a water-insoluble chitosan in the form of a powder having a grain size comprised between 0.1 and 200 micrometers, said water-insoluble chitosan being at a concentration from 1 to 10 g/hL of said liquid food to be treated.

### Claim 26 (depends on 25)

The method according to claim 25, wherein said liquid food is selected from the group consisting of a liquid food prepared by fermentation, a wine, a beer, a cider, a sparkling wine, and a fruit juice.

### Claim 27 (depends on 25)

The method according to claim 25 wherein said grain size is comprised between 5 and 50 micrometers.

### Claim 28

A method for curative, or preventive treatment of a liquid food of vegetable origin in which undesirable yeasts have to be removed, or the presence of undesirable yeasts has to be prevented, or the population of undesirable yeasts has to be limited, said method comprising putting liquid food in the presence with a water-insoluble chitosan or a composition comprising said chitosan, said chitosan being in the form of a powder having a grain size comprised between 0.1 and 200 micrometers, said chitosan being at a concentration from 1 to 10 g/hL of liquid food to be treated.

### Claim 29 (depends on 28)

The method according to claim 28, wherein the liquid food is a wine, a beer, or a cider, and wherein undesirable yeasts are B. anomalus, B. bruxellensis, or a mixture of B. anomalus and B. bruxellensis.

### Claim 30 (depends on 28)

The method according to claim 28, wherein said grain size is comprised between 5 and 50 micrometers.

### Claim 31 (depends on 28)

The method according to claim 28, wherein said liquid food is a liquid food of vegetable origin prepared by fermentation.

### Claim 32 (depends on 28)

The method according to claim 28, wherein said liquid food is put into the presence of chitosan for at least 3 days.

### Claim 33 (depends on 28)

The method according to claim 28, wherein said treatment is carried out on liquid food during fermentation.

### Claim 34 (depends on 28)

The method according to claim 28, wherein said chitosan or composition comprising said chitosan is separated from the liquid food for preserving the liquid food.