# US 2013/0156740 A1 · Bio-pestcide methods and compositions > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 2013/0156740 A1 - Document ID: US20130156740A1 - Status: Published application - Kind: A1 - Application number: 13/719,624 - Filed: 2012-12-19 - Published: 2013-06-20 - Earliest claimed: 2011-12-19 - Assignees: Novozymes Biologicals Holdings A/S - Inventors: Jarrod E. Leland - Web page: https://patentgrep.com/patent/US20130156740A1 ## CPC - A01N 63/30: Microbial fungi; Substances produced thereby or obtained therefrom - A01N 63/34: Aspergillus - A01N 63/38: Trichoderma - A01N: PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF (preservation of food or foodstuff A23); BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES (preparations for medical, dental or toiletry purposes which kill or prevent the growth or proliferation of unwanted organisms A61K); PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS ## Abstract The present invention is directed to the combination of biopesticide and at least one exogenous cuticle degrading enzymes (e.g., a protease, chitinase, lipase and/or cutinase) for controlling (preventing or eliminating) pests. The use of an exogenous cuticle degrading enzyme increases the efficacy of the biopesticide by increasing the speed and/or efficiency of infestation of the pest resulting in faster or more effective killing or disabling of the pest by the biopesticide. The present invention accordingly provides methods for controlling a pest comprising treating a pest habitat with a combination of pesticidally effective amounts of at least one biopesticide and at least one exogenous cuticle degrading enzyme. Pest control compositions are also described. ## Claims ### Claim at position 1 1-31. (canceled) ### Claim 32 A method for controlling a pest comprising treating a pest habitat with a biopesticide comprising a pesticidally effective amount of at least one fungal pesticide and at least one exogenously applied cuticle degrading enzyme. ### Claim 33 (depends on 32) The method of claim 32, wherein the at least one fungal pesticide comprises is at least one entomopathogenic fungus. ### Claim 34 (depends on 32) The method of claim 32, wherein the at least one fungal pesticide comprises at least one nematopathogenic fungus. ### Claim 35 (depends on 32) The method of claim 32, wherein the at least one fungal pesticide comprises at least one acaripathogenic fungus. ### Claim 36 (depends on 32) The method of claim 32, wherein the at least one fungal pesticide has one or more of the following properties: entomopathogenic activity, acaripathogenic activity, and nematopathogenic properties. ### Claim 37 (depends on 32) The method of claim 32, wherein the at least one fungal pesticide is selected from the group consisting of species of Ascomycota, Alternaria, Beauveria, Lecanicillium, Metarhizium, Verticillium, Trichoderma, Aspergillus, Nomuraea, Paecilomyces, Isaria, Hirsutella, Fusarium, Cordyceps, Entomophthora, Zoophthora, Pandora, Entomophaga, Entomophthorales and Zygomycota. ### Claim 38 (depends on 32) The method of claim 32, wherein the at least one fungal pesticide comprises Alternaria cassiae, Fusarium lateritum, Fusarium solani, Verticillium lecanii, Aspergillus parasiticus, Metarhizium anisopliae, Beauveria bassiana and any combination thereof, preferably Metarhizium anisopliae. ### Claim 39 (depends on 32) The method of claim 32, wherein the least one exogenously applied cuticle degrading enzyme is selected from the group consisting of protease, peptidase, chitinase, chitosanase, lipase, cutinase and any combination thereof. ### Claim 40 (depends on 32) The method of claim 32, wherein the treating comprises treating the habitat with a composition comprising the pesticidally effective amounts of the at least one fungal pesticide, at least one exogenously applied cuticle degrading enzyme and a carrier for the at least one fungal pesticide and the at least one exogenously applied cuticle degrading enzyme. ### Claim 41 (depends on 32) The method of claim 32, wherein the treating comprises treating the habitat with a composition comprising the at least one fungal pesticide and a carrier for the fungal pesticide and a composition comprising the at least one exogenously applied cuticle degrading enzyme and a carrier for the at least one exogenously applied cuticle degrading enzyme. ### Claim 42 (depends on 32) The method of claim 32, wherein the at least one cuticle degrading enzyme is in an amount effective to increase the boring through of fungal pesticide through the cuticle of the target pest. ### Claim 43 (depends on 32) The method of claim 32, wherein the habitat is a plant, soil, or water environment. ### Claim 44 A biopesticide comprising pesticidally effective amounts of at least one fungal pesticide and at least one exogenous cuticle degrading enzyme. ### Claim 45 (depends on 44) The biopesticide of claim 44, wherein the at least one fungal pesticide comprises is at least one entomopathogenic fungus. ### Claim 46 (depends on 44) The biopesticide of claim 44, wherein the at least one fungal pesticide comprises at least one nematopathogenic fungus. ### Claim 47 (depends on 44) The biopesticide of claim 44, wherein the at least one fungal pesticide comprises at least one acaripathogenic fungus. ### Claim 48 (depends on 44) The biopesticide of claim 44, wherein the at least one fungal pesticide has one or more of the following properties: entomopathogenic activity, acaripathogenic activity, and nematopathogenic activity. ### Claim 49 (depends on 44) The biopesticide of claim 44, wherein the at least one fungal pesticide is selected from the group consisting of species of Ascomycota, Alternaria, Beauveria, Lecanicillium, Metarhizium, Verticillium, Trichoderma, Aspergillus, Nomuraea, Paecilomyces, Isaria, Hirsutella, Fusarium, Cordyceps, Entomophthora, Zoophthora, Pandora, Entomophaga, Entomophthorales and Zygomycota. ### Claim 50 (depends on 44) The biopesticide of claim 44, wherein the at least one fungal pesticide comprises Alternaria cassiae, Fusarium lateritum, Fusarium solani, Verticillium lecanii, Aspergillus parasiticus, Metarhizium anisopliae, Beauveria bassiana and any combination thereof, more preferably, and Metarhizium anisopliae. ### Claim 51 (depends on 44) The biopesticide of claim 44, wherein the least one exogenous cuticle degrading enzyme is selected from the group consisting of protease, peptidase, chitinase, chitosanase, lipase and any combination thereof. ### Claim 52 (depends on 44) The biopesticide of claim 44, wherein the biopesticide comprises a composition comprising the pesticidally effective amounts of the at least one fungal pesticide, at least one exogenous cuticle degrading enzyme and a carrier for the at least one fungal pesticide and the at least one exogenous cuticle degrading enzyme. ### Claim 53 (depends on 44) The biopesticide of claim 44, wherein the biopesticide comprises a composition comprising the at least one fungal pesticide and a carrier for the fungal pesticide and a composition comprising the at least one exogenous cuticle degrading enzyme and a carrier for the cuticle degrading enzyme. ### Claim 54 (depends on 44) The biopesticide of claim 44, wherein the at least one cuticle degrading enzyme is in an amount effective to increase the penetration of the fungal pesticide through the cuticle of the target pest. ### Claim 55 (depends on 44) The biopesticide of claim 44, wherein the biopesticide is in the form of a wettable powder, dust, granule, bait, solution, emulsifiable concentrate, emulsion, suspensions, concentrate, spray, microparticle, microcapsules, topical treatment, gel, seed coating, systemic uptake, bait, eartag, bolus, or fogger.