# US 2013/0156645 A1 · C-reactive protein imprinted polymer film and microchip system utilizing the same > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 2013/0156645 A1 - Document ID: US20130156645A1 - Status: Published application - Kind: A1 - Application number: 13/715,693 - Filed: 2012-12-14 - Published: 2013-06-20 - Earliest claimed: 2011-12-16 - Assignees: National Tsing Hua University; Mackay Memorial Hospital - Inventors: Chien-Chong Hong; Szu-Ying Chen; Jia-Cherng Horng; Chie-Pein Chen - Web page: https://patentgrep.com/patent/US20130156645A1 ## CPC - G01N 33/545: Synthetic resin - G01N 2333/4737: C-reactive protein - Y10T 428/24479: including variation in thickness - G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q) - Y10T: TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION ## Abstract The present invention is a C-reactive protein imprinted polymer film. The C-reactive protein antibody imprinted polymer film comprises a plurality of imprinted nanocavities with unified orientation and distribution formed by removing a plurality of C-reactive proteins from a polymer film. Its ability to capture the target proteins can achieve 99% compared with the natural antibodies. The present invention further provides a C-reactive protein microchip system formed by the dynamic capacitance sensing method with the above imprinted polymer film. The C-reactive protein microchip system comprises a body having a first chamber and a second chamber, and a detector. ## Claims ### Claim 1 A C-reactive protein imprinted polymer film, comprising: a plurality of imprinted nanocavities with unified orientation and distribution formed by removing a plurality of C-reactive proteins from a polymer film, wherein the C-reactive proteins are bound to a plurality of antibodies on a modified surface of a first substrate. ### Claim 2 (depends on 1) The C-reactive protein imprinted polymer film of claim 1, wherein the modified surface of the first substrate is formed by a gold layer on the first substrate surface binding with cysteamine and glutaraldehyde in order. ### Claim 3 (depends on 2) The C-reactive protein imprinted polymer film of claim 2, wherein each antibody of the plurality of antibodies binds to an aldehyde group of the glutaraldehyde, which antibody does not bind to the glutaraldehyde when the aldehyde group is bound to glycine. ### Claim 4 (depends on 1) The C-reactive protein imprinted polymer film of claim 1, wherein the C-reactive proteins are connected to O-4-nitrophenylphosphoryl choline to form a plurality of precomposites which each micro-contacts with a composition on a second substrate, and the composition is consisted of a cross-linker and an initiator with the molar ratio of the cross-linker to the initiator from 600:1 to 640:1. ### Claim 5 (depends on 4) The C-reactive protein imprinted polymer film of claim 4, wherein the cross-linker is dimethacrylate, polyethylene glycol diacrylate or trimethylolpropane triacrylate; and the initiator is 2,2′-dimethoxy-2-phenyl acetophenone, 1-hydroxy-cyclohexyl-phenyl ketone, p-phenyl benzophenone or benzyl dimethyl ketal. ### Claim 6 A C-reactive protein antibody imprinted polymer film, comprising: a plurality of imprinted nanocavities with unified orientation and distribution formed by removing a plurality of C-reactive protein antibodies from a polymer film, wherein the C-reactive protein antibodies are bound to a modified surface of a first substrate. ### Claim 7 (depends on 6) The C-reactive protein antibody imprinted polymer film of claim 6, wherein the modified surface of the first substrate is formed by a gold layer on the first substrate surface binding with cysteamine and glutaraldehyde in order. ### Claim 8 (depends on 7) The C-reactive protein antibody imprinted polymer film of claim 7, wherein each antibody of the plurality of antibodies binds to an aldehyde group of the glutaraldehyde, which antibody does not bind to the glutaraldehyde when the aldehyde group is bound to glycine. ### Claim 9 (depends on 6) The C-reactive protein antibody imprinted polymer film of claim 6, wherein the C-reactive protein antibodies are connected to O-4-nitrophenylphosphorylcholine to form a plurality of precomposites which micro-contacts with a composition of a second substrate, and the composition is consisted of a cross-linker and an initiator with the molar ratio of the cross-linker to the initiator from 600:1 to 640:1. ### Claim 10 (depends on 9) The C-reactive protein antibody imprinted polymer film of claim 9, wherein the cross-linker is dimethacrylate, polyethylene glycol diacrylate or trimethylolpropane triacrylate; and the initiator is 2,2′-dimethoxy-2-phenyl acetophenone, 1-hydroxy-cyclohexyl-phenyl ketone, p-phenyl benzophenone or benzyl dimethyl ketal. ### Claim 11 (depends on 1) A C-reactive protein microchip system comprising: a body, comprising: a first chamber including a C-reactive protein imprinted polymer film having a plurality of imprinted nanocavities of claim 1 connected to a first portal and a second portal respectively, when a sample comprising C-reactive proteins is injected into the first portal and flowing through the C-reactive protein imprinted polymer film, flowing out of the first chamber from the second portal; and a second chamber including a sensing electrode connected to a third portal and a fourth portal respectively, when an extracting solvent is injected into the first portal to extract the C-reactive proteins being captured by the imprinted nanocavities, flowing into the second chamber via the third portal, flowing out of the second chamber from the fourth portal; and a detector, for detecting a potential change of the sensing electrode and generating a detecting result based on the detected potential change. ### Claim 12 (depends on 11) The C-reactive protein microchip system of claim 11, wherein the sensing electrode is a gold electrode. ### Claim 13 (depends on 11) The C-reactive protein microchip system of claim 11, wherein the sensing electrode is a finger electrode. ### Claim 14 (depends on 11) The C-reactive protein microchip system of claim 11, wherein the finger electrode is formed by a gold-plated plastic material processing photolithograph. ### Claim 15 (depends on 13) The C-reactive protein microchip system of claim 13, wherein the sensing electrode has a vulcanized surface or an antibody modified surface. ### Claim 16 (depends on 15) The C-reactive protein microchip system of claim 15, wherein the antibody is a biological antibody. ### Claim 17 (depends on 11) The C-reactive protein microchip system of claim 11, wherein the body is in the form of flat-shaped. ### Claim 18 (depends on 11) The C-reactive protein microchip system of claim 11, wherein the potential change is caused by dynamic balancing relationship between potential energy of an equivalent circuit and a sensing circuit of the detector, and the potential change is related to an amount of the C-reactive proteins contained in the extracting solvent, wherein the equivalent circuit is formed with the sensing electrode and the extracting solvent in the second chamber. ### Claim 19 (depends on 11) The C-reactive protein microchip system of claim 11, wherein the detecting result is determined by a time coefficient of the potential change, and total detection time is less than 110 seconds. ### Claim 20 (depends on 6) A C-reactive protein microchip system comprising: a body, comprising: a first chamber including a C-reactive protein antibody imprinted polymer film having a plurality of imprinted nanocavities of claim 6 connected to a first portal and a second portal respectively, when a sample comprising C-reactive proteins is injected into the first chamber and flowing through the C-reactive protein antibody imprinted polymer film, flowing out of the first chamber from the second portal; and a second chamber including a sensing electrode connected to a third portal and a fourth portal respectively, when an extracting solvent is injected into the first portal to extract the C-reactive proteins being captured by the imprinted nanocavities, flowing into the second chamber via the third portal, flowing out of the second chamber from the fourth portal; and a detector, for detecting a potential change of the sensing electrode and generating a detecting result based on the detected potential change.