# US 2013/0152349 A1 · Method for Making a Nano-Composite Gas Sensor > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 2013/0152349 A1 - Document ID: US20130152349A1 - Status: Published application - Kind: A1 - Application number: 13/328,115 - Filed: 2011-12-16 - Published: 2013-06-20 - Earliest claimed: 2011-12-16 - Assignees: Chung-Shan Institute of Science and Technology, Armaments, Bureau, Ministry of National Defense - Inventors: Li-Chun Wang; Tseng-Hsiung Su; Shang-Ren Yang; Cheng-Long Ho; Han-Wen Kuo; Kea-Tiong Tang - Web page: https://patentgrep.com/patent/US20130152349A1 ## CPC - G01N 27/127: {comprising nanoparticles} - B82Y 15/00: Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors - G01N 27/12: of a solid body in dependence upon absorption of a fluid; of a solid body in dependence upon reaction with a fluid {, for detecting components in the fluid} - Y10T 29/49124: On flat or curved insulated base, e.g., printed circuit, etc. - G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q) - B82Y: SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES - Y10T: TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION ## Abstract There is disclosed a method for making a nano-composite gas sensor. At first, there is provided a substrate. Then, electrodes are provided on the substrate in an array. Finally, a gas-sensing membrane is provided on the electrodes. The gas-sensing membrane includes a nano-conductive film and a peptide film. ## Claims ### Claim 1 A method for making a nano-composite gas sensor including the steps of: providing a substrate 1; providing electrodes 2 on the substrate in an array; and providing a gas-sensing membrane 3 on the electrodes 2, wherein the gas-sensing membrane 3 includes a nano-conductive film 31 and a peptide film 32. ### Claim 2 (depends on 1) The method for making a nano-composite gas sensor according to claim 1, wherein the substrate includes an insulating material coated on a material selected from the group consisting of metal, semiconductor and ceramic materials. ### Claim 3 (depends on 2) The method for making a nano-composite gas sensor according to claim 2, wherein the semiconductor is provided in the form of a silicon wafer. ### Claim 4 (depends on 1) The method for making a nano-composite gas sensor according to claim 1, wherein the electrodes are made of a material selected from the group consisting of gold, platinum, silver and aluminum. ### Claim 5 (depends on 1) The method for making a nano-composite gas sensor according to claim 1, wherein the electrodes are made of a configuration selected from the group consisting of fork-shaped, elongated and helical. ### Claim 6 (depends on 1) The method for making a nano-composite gas sensor according to claim 1, wherein the peptide film 32 is provided on the nano-conductive film 31. ### Claim 7 (depends on 1) The method for making a nano-composite gas sensor according to claim 1, wherein the nano-conductive film 31 is made of a material selected from the group consisting of carbon nanotubes, carbon black, carbon balls and graphene. ### Claim 8 (depends on 7) The method for making a nano-composite gas sensor according to claim 7, wherein the carbon nanotubes are evenly dispersed in a solvent selected from the group consisting of MEK, IPA, H2O and PVA to provide a carbon nanotube-based mixture of 0.1 wt % to 2.0 wt % of carbon nanotubes. ### Claim 9 (depends on 7) The method for making a nano-composite gas sensor according to claim 7, wherein the carbon nanotubes are evenly dispersed in a solvent selected from the group consisting of MEK, IPA, H2O and PVA to provide a carbon nanotube-based mixture that includes 0.5 wt % to 1.0 wt % of carbon nanotubes. ### Claim 10 (depends on 1) The method for making a nano-composite gas sensor according to claim 1, wherein the peptide film 32 is made of a material selected from the group consisting of Beta-Amyloid (1-40), Human galectin-1 and Human albumin. ### Claim 11 (depends on 10) The method for making a nano-composite gas sensor according to claim 10, wherein the peptide film 32 is made by evenly dispersing peptide in a solvent selected from the group consisting of Toluene, THF and H2O to provide a peptide-based mixture that includes 5 wt % to 50 wt % of peptide. ### Claim 12 (depends on 10) The method for making a nano-composite gas sensor according to claim 10, wherein the peptide film 32 is made by evenly dispersing peptide in a solvent selected from the group consisting of Toluene, THF and H2O to provide a peptide-based mixture that includes 10 wt % to 45 wt % of peptide. ### Claim 13 (depends on 1) The method for making a nano-composite gas sensor according to claim 1, wherein the step of providing the gas-sensing membrane 3 includes the steps of: providing the nano-conductive film 31 by evenly dispersing carbon nanotubes in a solvent to provide a carbon nanotube-based mixture; providing the peptide film 32 by evenly dispersing peptide in the solvent to provide a peptide-based mixture; evenly providing 1 to 3 μL of the carbon nanotube-based mixture on the electrodes 2; drying the carbon nanotube-based mixture in a vacuum oven at 50 to 70 degrees Celsius for 1 to 3 hours to form the nano-conductive film 31 with a resistance of 1 to 10 KΩ; evenly providing 1 to 3 μl of the peptide-based mixture on the nano-conductive film 31; and drying the peptide-based mixture in a vacuum oven at 50 to 60 degrees Celsius for 1 to 4 hours to form the peptide film. ### Claim 14 (depends on 13) The method for making a nano-composite gas sensor according to claim 13, wherein the solvent is selected from the group consisting of MEK, IPA, H2O and PVA. ### Claim 15 (depends on 1) The method for making a nano-composite gas sensor according to claim 1, wherein the step of providing the gas-sensing membrane 3 includes the steps of: providing the nano-conductive film 31 by evenly dispersing carbon nanotubes in a solvent to provide a carbon nanotube-based mixture; providing the peptide film 32 by evenly dispersing peptide in the solvent to provide a peptide-based mixture; evenly providing 1 to 10 μL of the carbon nanotube-based mixture on the electrodes 2; drying the carbon nanotube-based mixture in a vacuum oven at 40 to 80 degrees Celsius for 1 to 5 hours to form the nano-conductive film 31 with a resistance of 1 to 50 KΩ; evenly providing 1 to 10 μl of the peptide-based mixture on the nano-conductive film 31; and drying the peptide-based mixture in a vacuum oven at 40 to 60 degrees Celsius for 1 to 5 hours to form the peptide film. ### Claim 16 (depends on 15) The method for making a nano-composite gas sensor according to claim 15, wherein the solvent is selected from the group consisting of MEK, IPA, H2O and PVA.