Organic peroxide compounds for microorganism inactivation
AssigneeNovartis AG
InventorsJens Peter Gregersen, Thomas Grundemann
Life of this patent
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Abstract
Multifunctional organic peroxides are used as microbiological inactivators and/or for degrading nucleic acids. These include at least one carbon atom and at least two organic peroxide groups. The inactivator is ideally a hydroperoxide. The invention is particularly useful during preparation of viral vaccines.
Claims41
1-40. (canceled)
A process for treating a microorganism-containing sample, or for treating a sample which is at risk of being microorganism-containing, comprising contacting the sample with a multifunctional organic peroxide having formula I:
- where:
- R1a is H, C1-6alkyl, C2-6alkenyl, C1-6alkynyl, C1-6-alkoxy, C2-6-alkenyloxy, C(O)H, C(O)C1-6alkyl, C(O)OC1-6alkyl, optionally N-mono or N-di C1-6alkylated amino, optionally N-mono or N-di C1-6alkylated aminocarbonyl, S(O)0-2C1-6alkyl, heterocycloalkyl, aryl or heteroaryl;
- R2a is H, C1-6alkyl, C2-6alkenyl, C1-6alkynyl, C1-6-alkoxy, C2-6-alkenyloxy, C(O)H, C(O)C1-6alkyl, C(O)OC1-6alkyl, optionally N-mono or N-di C1-6alkylated amino, optionally N-mono or N-di C1-6alkylated aminocarbonyl, S(O)0-2C1-6alkyl, heterocycloalkyl, aryl or heteroaryl, —(CR4R5)nCR6(OOH)2, or R2a is linked to R2b by L;
- R3 is H or C(OOH)R1bR2b,
- R1b is H, C1-6alkyl, C2-6alkenyl, C1-6alkynyl, C1-6-alkoxy, C2-6-alkenyloxy, C(O)H, C(O)C1-6alkyl, C(O)OC1-6alkyl, optionally N-mono or N-di C1-6alkylated amino, optionally N-mono or N-di C1-6alkylated aminocarbonyl, S(O)0-2C1-6alkyl, heterocycloalkyl, aryl or heteroaryl,
- R2b is H, C1-6alkyl, C2-6alkenyl, C1-6alkynyl, C1-6-alkoxy, C2-6-alkenyloxy, C(O)H, C(O)C1-6alkyl, C(O)OC1-6alkyl, optionally N-mono or N-di C1-6alkylated amino, optionally N-mono or N-di C1-6alkylated aminocarbonyl, S(O)0-2C1-6alkyl, heterocycloalkyl, aryl or heteroaryl, or R2b is linked to R2a by L;
- R4 is, at each occurrence, selected from H or C1-3 alkyl, hydroxyl, cyano, nitro, C2-4-alkenyl, C1-3-alkoxy, C2-4-alkenyloxy, C1-3-alkylcarbonyl, carboxy, C1-6-alkoxycarbonyl, optionally N-mono or N-di C1-3alkylated aminocarbonyl, C1-3-thioalkyl, C1-3-alkylsulfinyl, C1-3-alkylsulfonyl, C1-3-alkylaminosulfonyl and di-C1-3-alkylaminosulfonyl;
- R5 is, at each occurrence, selected from H or C1-3 alkyl, hydroxyl, cyano, nitro, C2-4-alkenyl, C1-3-alkoxy, C2-4-alkenyloxy, C1-3-alkylcarbonyl, carboxy, C1-6-alkoxycarbonyl, optionally N-mono or N-di C1-3alkylated aminocarbonyl, C1-3-thioalkyl, C1-3-alkylsulfinyl, C1-3-alkylsulfonyl, C1-3-alkylaminosulfonyl and di-C1-3-alkylaminosulfonyl;
- L is C1-8alkylene;
- R6 is H, C1-6alkyl, C2-6alkenyl, C1-6alkynyl, C1-6-alkoxy, C2-6-alkenyloxy, C(O)H, C(O)C1-6alkyl, C(O)OC1-6alkyl, optionally N-mono or N-di C1-6alkylated amino, optionally N-mono or N-di C1-6alkylated aminocarbonyl, S(O)0-2C1-6alkyl, heterocycloalkyl, aryl or heteroaryl;
- n is 1 to 8.
A process for inactivating viruses in a virus-containing sample, or a sample which is at risk of being virus-containing, comprising contacting the sample with a multifunctional organic peroxide that does not include a —C(═O)—OOH group.
A process for treating a microorganism-containing sample, or for treating a sample which is at risk of being microorganism-containing, comprising contacting the sample with a multifunctional organic peroxide that does not include a —C(═O)—OOH group, wherein the process also involves a step in which an alkylating agent is used.
A process for treating a sample which contains nucleic acids, comprising contacting the sample with a multifunctional organic peroxide that does not include a —C(═O)—OOH group to degrade the nucleic acids.
A method for preparing a pharmaceutical composition, comprising steps of:
- (i) contacting a microorganism-containing sample with a multifunctional organic peroxide that does not include a —C(═O)—OOH group to inactivate microorganisms therein;
- (ii) preparing a pharmaceutical composition from the product of step (i).
An aqueous solution of a multifunctional organic peroxide having formula I, wherein the multifunctional organic peroxide is present at a concentration of either (a) less than 1% by weight or (b) between 5-70% by weight.
An aqueous composition comprising (i) inactivated viruses and (ii) less than 1% by weight of a multifunctional organic peroxide that does not include a —C(═O)—OOH group.
An aqueous composition comprising the reaction product of a virus and a multifunctional organic peroxide that does not include a —C(═O)—OOH group.
Dependencies are inferred from the claim text.