Non-Fluorescent Quencher Compounds and Biomolecular Assays
AssigneeLife Technologies Corporation
InventorsGregory J. Ewing, Deborah Kay Fieri, Khairuzzaman Bashar Mullah, Ronald Graham
Life of this patent
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Abstract
Bis-diazo, triaryl and aryldiazo-N-arylphenazonium quencher moieties, substituted with electron-withdrawing and electron-donating substituents which induce polarity in the delocalized aryl/diazo ring systems, are useful as labels when attached to biomolecules such as polynucleotides, nucleosides, nucleotides and polypeptides. The quencher moieties are non-fluorescent and accept energy transfer from fluorescent reporter labels by any energy-transfer mechanism, such as FRET. Fluorescence quencher compositions are useful in preparing quencher labeled biomolecules for various molecular biology assays based on fluorescence detection.
Claims31
A compound comprising:
- (i) a quencher moiety comprising structure (Q):
- wherein:
- at least one aryl carbon of (Q) is substituted with an electron-withdrawing group;
- and
- at least one aryl carbon of (Q) is substituted with an electron-donating group;
- and
- (ii) an optionally protected reactive linking group linked to an aryl carbon of the quencher moiety, either directly or by way of an optional linker moiety.
3-54. (canceled)
A compound comprising:
- (i) a quencher moiety comprising structure (Q1), wherein Q1 comprises the following structure:
- wherein:
- R′, R2, R4, R5, R6, R7, R8, R9, R10, R11, R12, R13 and R14 are each, independently of one another, selected from hydrogen, an electron-withdrawing group and an electron-donating group, or, alternatively R4 may be taken together with R5 or R6 may be taken together with R7 to form a benzo group,
- wherein:
- (i) at least one of R1, R2, R4, R5, R6, R7, R8, R9, R10, R11, R12, R13 or R14 is an electron-withdrawing group;
- and (ii) at least one of R1, R2, R4, R5, R6, R7, R8, R9, R10, R11, R12, R13 or R14 is an electron-donating group;
- and
- (ii) an optionally protected reactive linking group linked to an aryl carbon of the quencher moiety, either directly or by way of an optional linker moiety.
Dependencies are inferred from the claim text.