Acyltransferases and Uses Thereof in Fatty Acid Production

A polynucleotide comprising a nucleic acid sequence elected selected from the group consisting of:

• a) the nucleic acid sequence of SEQ ID NOs:
• 52, 1, 4, 7, 10, 13, 16, 19, 22, 25, 28, 31, 34, 37, 40, 43, 46, 49, and 55;
• b) a nucleic acid sequence encoding the amino acid sequence of SEQ ID NO:
• 53, 2, 5, 8, 11, 14, 17, 20, 23, 26, 29, 32, 35, 38, 41, 44, 47, 50, or 56;
• c) a nucleic acid sequence being at least 40% identical to the nucleic acid sequence of a) or b), wherein said nucleic acid sequence encodes a polypeptide having acyltransferase activity;
• d) a nucleic acid sequence encoding a polypeptide having acyltransferase activity and having an amino acid sequence which is at least 45% identical to the amino acid sequence of b);
• and
• e) a nucleic acid sequence which is capable of hybridizing under one of the following sets of conditions to any one of a) to d), wherein said nucleic acid sequence encodes a polypeptide having acyltransferase activity:
• i) hybridization in 50 mM Tris, pH 7.6, 6×SSC, 5× Denhardt's, 1.0% sodium dodecyl sulfate (SDS), 100 μg denaturated calf thymus DNA at 34° C. overnight and wash twice with 2×SSC, 0.5% SDS at room temperature for 15 min each, repeat twice with 0.2×SSC, 0.5% SDS at room temperature for 15 min each and then repeat twice with 0.2 SSC, 0.5% SDS at 50° C. for 15 min;
• ii) hybridization in 6×SSPE (Sodium chloride Sodium Phosphate-EDTA), 5× Denhardt's solution, 0.5% SDS, 100 μg denaturated calf thymus DNA at 34° C. overnight and wash twice with 2×SSC, 0.5% SDS at room temperature for 15 min each, repeat twice with 0.2×SSC, 0.5% SDS at room temperature for 15 min each and then repeat twice with 0.2 SSC, 0.5% SDS at 50° C. for 15 min;
• iii) hybridization in 20-30% formamide, 5×SSPE, 5× Denhardt's solution, 1% SDS 100 μg denaturated salmon sperm DNA at 34° C. overnight and wash twice with 2×SSPE, 0.2% SDS at 42° C. for 15 min each, repeat twice with 2×SSPE, 0.2% SDS at 55° C. for 30 min each and repeat twice with 0.2 SSC, 0.5% SDS at 50° C. for 15 min;
• iv) hybridization in 7% SDS, 0.5 M NaPO4, 1 mM EDTA at 50° C. overnight and wash in 2×SSC, 0.1% SDS at 50° C. or 65° C.;
• v) hybridization in 7% SDS, 0.5 M NaPO4, 1 mM EDTA at 50° C. overnight and wash in 1×SSC, 0.1% SDS at 50° C. or 65° C.;
• or
• vi) hybridization in 7% SDS, 0.5 M NaPO4, 1 mM EDTA at 50° C. overnight and wash in 0.1×SSC, 0.1% SDS at 50° C. or 65° C.