Pest-resistant plants containing a combination of a spider toxin and a chitinase
AssigneeFuturagene Israel Ltd.
InventorsDror Avisar, Hanan Stein, Ziv Shani
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Abstract
An isolated polynucleotide is disclosed comprising: (i) a nucleic acid sequence encoding at least one toxic peptide, the toxic peptide being a spider toxin; and (ii) a nucleic acid sequence encoding a chitinase attached to a secretion signal sequence. Uses thereof and plants expressing same are also disclosed.
Claims34
An isolated polynucleotide comprising:
- (i) a nucleic acid sequence encoding at least one toxic peptide, said toxic peptide being a spider toxin;
- and
- (ii) a nucleic acid sequence encoding a chitinase attached to a secretion signal sequence.
An isolated polynucleotide comprising:
- (i) a nucleic acid sequence encoding at least one toxic peptide, said toxic peptide not being chitinase;
- and
- (ii) a nucleic acid sequence encoding a chitinase.
A nucleic acid construct system comprising:
- (i) a first nucleic acid construct comprising an isolated polynucleotide which comprises a nucleic acid sequence encoding a toxic peptide and a cis regulatory element;
- and
- (ii) a second nucleic acid construct comprising an isolated polynucleotide which comprises a nucleic acid sequence encoding a chitinase and a cis regulatory element.
(canceled)
22-25. (canceled)
28-31. (canceled)
34-35. (canceled)
An insecticidal composition comprising:
- (i) a toxic peptide;
- and
- (ii) a chitinase.
(canceled)
A method of controlling or exterminating an insect, the method comprising expressing in a host plant of said insect an isolated polynucleotide which comprises a nucleic acid sequence encoding a toxic peptide selected from the group consisting of SEQ ID NOs:
- 9, 15, 24, 30 and 55-57.
41-45. (canceled)
Dependencies are inferred from the claim text.