New Method of Using N-Thio Compounds for Oligonucleotide Synthesis
AssigneeGirindus America, Inc.
InventorsYigang He, Victor Sorokin, Wieslaw Adam Mazur
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Abstract
A method for synthesizing an oligonucleotide which comprises using a sulfurizing agent of general formula (I) for sulfurizing at least one phosphorus internucleotide linkage of a precursor of the oligonucleotide, wherein R is an aryl group or a heteroaryl group, which is bonded to the S-atom through an annular carbon atom; and R1 and R2 are independently organic residues, preferably a C1-C20 hydrocarbon residue. The method may further comprise purifying the oligonucleotide. Also included is a process for the synthesis of the sulfurizing agent.
Claims25
A method for synthesizing an oligonucleotide which comprises using a sulfurizing agent of general formula (I):
- wherein
- R is selected from a group consisting of an aryl group and a heteroaryl group;
- which is bonded to the S-atom through an annular carbon atom;
- R1 and R2 are independently organic residues, preferably a C1-C20, optionally unsaturated or aromatic, hydrocarbon residue;
- for sulfurizing at least one phosphorus internucleotide linkage of a precursor of said oligonucleotide.
Dependencies are inferred from the claim text.