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AssigneeKaneka Corporation
InventorsShinichi Yoshida, Dai Murata, Shunichi Taira, Masayuki Takano, Keita Iguchi, Yoshiyuki Nakano
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An object of the present invention is to create a novel engineered Protein A ligand having better antibody dissociation properties in the acidic condition compared with known engineered Protein A ligands. The present invention provides a protein having an affinity for an immunoglobulin, including an amino acid sequence obtained by introducing, into an amino acid sequence derived from any of E, D, A, B and C domains of Protein A, at least one amino acid substitution at any one or more of amino acid residues corresponding to positions 31 to 37 of the A, B and C domains (positions 29 to 35 of the E domain, positions 34 to 40 of the D domain), which are conserved in all the domains, the protein having a lower affinity for an Fab region of an immunoglobulin than a protein having the amino acid sequence before introduction of the substitution.
A protein having an affinity for an immunoglobulin, comprising an amino acid sequence obtained by introducing, into an amino acid sequence derived from at least one domain selected from E, D, A, B and C domains of Protein A of SEQ ID Nos:1 to 5, at least one amino acid substitution at any one or more of amino acid residues at positions 31 to 37 of the A, B and C domains, amino acid residues at positions 29 to 35 of the E domain, and amino acid residues at positions 34 to 40 of the D domain,
Dependencies are inferred from the claim text.