Multiplexed in vivo screening of biological samples
AssigneeThe Trustees of Columbia University in the City of New York
InventorsSamuel K. Sia, HESAM PARSA
Life of this patent
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Abstract
Microfabricated platforms can be used to study a heterogeneous panel of biosamples in a realistic in vivo setting. The platform can be formed of a polymer (e.g., a hydrogel) and can be constructed for implantation into an animal host for in vivo testing. The platform can have a plurality of testing regions therein that are constructed to allow exposure of the testing region to the host stroma when implanted in vivo. For example, the microfabricated platform can be used for screening different cancer cell-lines (e.g., to identify which cell line responds to an anti-cancer drug) or for screening different biomaterials (e.g., to identify a composition with ideal host response for a specific implantable device).
Claims26
1-13. (canceled)
A device for screening multiple biological samples in vivo, the device comprising:
- a platform member having a plurality of testing regions thereon, each of the testing regions being configured to hold a different biological sample for interaction in vivo with stroma cells when implanted into a host animal,
- the platform member including isolating portions arranged between adjacent testing regions such that fluid and/or material cannot pass from one testing region to another testing region without contacting the host animal stroma cells when implanted into the host animal.
(canceled)
A system for screening multiple biological samples in vivo, the system comprising:
- a screening device including a platform with a plurality of testing regions thereon, each of the testing regions being configured to hold a different biological sample for interaction in vivo with stroma cells when implanted into a host animal, the platform further include isolating portions arranged between adjacent testing regions such that fluid and/or material can only pass from one testing region to another testing region by contacting the host animal stroma cells when implanted into the host animal;
- and
- an evaluation device configured to image the testing regions ex vivo so as to determine the effect of the in vivo exposure on the biological samples in the platform.
A method of culturing biological samples, comprising:
- arranging biologically varied cells or tissue cultures in an array that spans a curvilinear planar region;
- exposing the varied cells or tissue cultures to living tissue of a host animal such that the cells or tissue cultures receive chemical signals from the living tissue;
- regulating or perturbing the natural state of the host;
- and
- detecting an effect of the regulating or perturbing on the cells or tissue cultures.
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33-36. (canceled)
41-46. (canceled)
Dependencies are inferred from the claim text.