Generation of binding molecules

A method for producing a defined population of binding molecules, said method comprising:

• a) providing a population of B cells expressing a limited VL repertoire wherein essentially all of said B cells carry at least one VL,
• b) obtaining nucleic acids from said B cells,
• c) obtaining sequences of essentially all obtained nucleic acids of step b),
• d) performing a frequency analysis of the sequences from step c),
• e) selecting desired VH sequences,
• f) providing a host cell with at least one vector comprising at least one of said desired VH sequences and/or at least one VL sequence of said limited VL repertoire,
• g) culturing said host cells and allowing for expression of VH and/or VL polypeptides, and
• h) obtaining said binding molecules.