HtSNPs FOR DETERMINING A GENOTYPE OF CYTOCHROME P450 1A2, 2A6 AND 2D6, PXR AND UDP-GLUCURONOSYLTRANSFERASE 1A GENE AND MULTIPLEX GENOTYPING METHODS USING THEREOF

A method of determining a genotype of a human CYP2D6 gene, the method comprising:

• (a) collecting a biological sample from humans;
• (b) extracting nucleic acid from the sample collected at operation (a);
• (c) performing PCR with a primer which amplifies a human CYP2D6 gene or a fragment thereof by using the nucleic acid extracted at operation (b) as a template;
• and
• (d) determining a presence of at least 11 variants in a CYP2A6 gene including one from −1426C>T, 100C>T and 1039C>T;
• one from −1028T>C, −377A>G, 3877G>A, 4388C>T and 4401C>T;
• one from −740C>T, −678G>A, 214G>C, 221C>A, 223C>G, 227T>C, 232G>C, 233A>C, 245A>G and 2850C>T;
• 1611T>A;
• 1758G>A;
• 1887insTA;
• 2573insC;
• 2988G>A;
• 4125-4133insGTGCCCACT;
• 2D6 deletion;
• and 2D6 duplication.

A method of determining a human CYP2D6 gene by using a gene chip, the method comprising:

• (a) extracting a gene to be investigated and performing multiplex PCR to receive a PCR product having a SNP circumference to be identified;
• (b) performing ASPE reaction to an ASPE (allele specific primer extension) primer to identify a specific base of each allele;
• (c) mixing the reactant to the gene chip;
• and
• (d) analyzing the chip.