Closed-cycle continuous flow separators, systems and methods for the continuous isolation of target cells
AssigneeEmpire Technology Development LLC
InventorEzekiel Kruglick
Life of this patent
Estimating the term…
Abstract
Provided are closed-cycle continuous flow separator systems for isolating target cell populations, which comprises in operable connection, a binding area for binding a target cell to a binding particle; a first continuous flow centrifuge for separating target cell bound binding particles from non-target cells; a de-binding area for de-binding the target cell from the binding particle; and a second continuous flow centrifuge for separating the target cell from the binding particle.
Claims31
A closed-cycle continuous flow separator for isolating a target cell population, the closed-cycle continuous flow separator comprising:
- a binding area configured to bind binding particles to respective target cells of a cell mixture to form particle-bound cells;
- a first continuous flow centrifuge connected to the binding area and configured to separate unbound cells from the binding particles and the particle-bound cells;
- a de-binding area connected to the first continuous flow centrifuge and configured to dissociate the particle-bound cells into unbound particles and unbound target cells;
- and
- a second continuous flow centrifuge connected to the de-binding area and configured to separate the unbound particles and the unbound target cells.
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26-35. (canceled)
A system for separating a target cell from a mixed cell population, the system comprising:
- a binding particle for specifically binding to a target cell, the binding particle comprising a high density material and a binding agent;
- and
- a closed-cycle continuous flow separator configured to isolate the target cell from the mixed cell population, the closed-cycle continuous flow separator comprising:
- a binding area configured to bind the binding particle to the target cell of the mixed cell population to form a particle-bound cell;
- a first continuous flow centrifuge connected to the binding area and configured to separate unbound cells from the binding particle and the particle-bound cell;
- a de-binding area connected to the first continuous flow centrifuge and configured to dissociate the particle-bound cell into an unbound particle and an unbound target cell;
- and
- a second continuous flow centrifuge connected to the de-binding area and configured to separate the unbound particle and the unbound target cell.
37-63. (canceled)
Dependencies are inferred from the claim text.