US 2013/0095571 A1 · THE METHOD FOR QUICK AND SIMULTANEOUS DETERMINATION OF 16 INORGANIC ANIONS AND ORGANIC ACIDS IN TOBACCO

Abstract

The present invention discloses a method for quick and simultaneous determination of 16 inorganic anions and organic acids in tobacco by ion chromatography The retention behavior of inorganic anions and organic acids on the anion exchange column was investigated using potassium hydroxide produced by EGC-II KOH eluent autogenerator as eluent. The optimized gradient elution condition was obtained. The samples were prepared through extraction, filtration and dilution before analysis. The separation was performed on an anion exchange column. The time of the gradient elution program was 50 mins. Under the optimized conditions, the calibration of peak area for all the analytes were linear in the ranges of 105. The method in the present invention has the advantadges of simplicity, rapidity and accuracy, and is able to simultaneously determine 16 inorganic anions and organic acids in tobacco by one single time of injection.

Claims3

Dependent claims

A method for quick and simultaneous determination of 16 inorganic anions and organic acids in tobacco by ion chromatography, wherein the elution process comprises:

Concentration
Time
of KOH
(min)
(p/mmol · L−1)
Curve
Description
0.000
1
5
Start of analysis
8.000
1
5
Analysis of weak retention components
28.000
30
5
Analysis of medium retention
components
35.000
50
5
Analysis of strong retention components
40.000
50
5
Purge system
40.001
1
5
Balance system
50.000
1
5
End of analysis
conditions of said ion chromatograghy being:
anion exchange column:
IonPacAS11-HC (2 mm×50 mm) and IonPacAG11-HC (2 mm×250 mm);
column temperature:
30° C.;
flow rate:
0.30 ml/min;
injection volume:
25 μL;
suppressor current:
50 mA.

44 more elements

Dependencies are inferred from the claim text.

Facts

Application

13/806,115Filed 7 Dec 2011

Priority

CN 201010577905.78 Dec 2010

Published

18 Apr 2013

Term ends

Not estimatedThe source records don’t support an estimate.

Classification11 codes

G01N 33/0098
Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00: plants or trees
G01N 30/88
Column chromatography: integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
G01N 30/96
Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g.…:…
G01N 2030/884
Analysis specially adapted for the sample: organic compounds

The method for quick and simultaneous determination of 16 inorganic anions and organic acids in tobacco

Life of this patent

The method for quick and simultaneous determination of 16 inorganic anions and organic acids in tobacco

Life of this patent

Abstract

Claims3