Method for preparing 3-hydroxypropionic acid from glycerol in high yield
AssigneeKorea Research Institute of Bioscience and Biotech
InventorsChul Ho Kim, Jeong-Woo Seo, Lianhua Luo
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Abstract
The present invention relates to a method for producing 3-hydroxypropionic acid by culturing in a glycerol-containing medium a mutant microorganism obtained by inserting or amplifying a gene encoding propanediol utilization protein in a microorganism having the abilities to produce coenzyme B12 and produce 3-hydroxypropionic acid using glycerol as a carbon source According to the present invention, 3-hydroxypropionic acid can be produced in high yield from glycerol without having to add expensive coenzyme B12 as a cofactor.
Claims13
A mutant microorganism obtained by introducing or amplifying a gene encoding propanediol utilizing protein in a microorganism having the abilities to produce coenzyme B12 and produce 3-hydroxypropionic acid using glycerol as a carbon source.
A method for preparing a mutant microorganism having a high ability to produce 3-hydroxypropionic acid, comprising introducing or amplifying a gene encoding propanediol utilization protein in a microorganism having the abilities to produce coenzyme B12 and produce 3-hydroxypropionic acid using glycerol as a carbon source.
A Klebsiella pneumoniae mutant obtained by deleting a glycerol dehydrogenase gene (DhaD), a transcriptional activator gene (DhaR), a 1,3-propanediol oxidoreductase gene (DhaT) and a glycerol dehydratase reactivation factor II gene (DhaBA2) from Klebsiella pneumoniae and introducing a 1,3-propanediol oxidoreductase-encoding gene, an aldehyde dehydrogenase-encoding gene and a gene encoding propanediol utilization protein into the Klebsiella pneumoniae.
A method for preparing a Klebsiella pneumoniae mutant, the method comprising deleting a glycerol dehydrogenase gene (DhaD), a transcriptional activator gene (DhaR), a 1,3-propanediol oxidoreductase gene (DhaT) and a glycerol dehydratase reactivation factor II gene (DhaBA2) from Klebsiella pneumoniae and introducing a 1,3-propanediol oxidoreductase-encoding gene, an aldehyde dehydrogenase-encoding gene and a gene encoding propanediol utilization protein into the Klebsiella pneumoniae.
Dependencies are inferred from the claim text.