Microorganism for quantifying homocysteine, and use thereof
AssigneeLabgenomics Co., Ltd.
InventorsHyun Gyu Park, Min-Ah Woo, Moon Il Kim, Sang-Joon Hwang, Dae-Yeon Cho
Life of this patent
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Abstract
Provided are a microorganism for use in quantification of homocysteine and methionine and a method of quantifying homocysteine and methionine in a sample by using the microorganism.
Claims17
A method of quantifying homocysteine in a sample by using auxotrophic mutants of Escherichia coli, the method comprising:
- measuring the content of homocysteine and methionine in the sample by culturing homocysteine and methionine auxotrophic mutants of Escherichia coli in the sample,
- measuring the content of methionine in the sample by culturing methionine auxotrophic mutants of Escherichia coli in the sample, and
- determining the content of homocysteine by subtracting the content of methionine from the content of homocysteine and methionine in the sample.
Escherichia coli deposited under the Accession No. KCCM11069P that is homocysteine and methionine auxotroph and expresses luciferase.
Escherichia coli deposited under the Accession No. KCCM11070P that is methionine auxotroph and expresses luciferase.
A composition for quantifying homocysteine, the composition comprising Escherichia coli MetB− and Escherichia coli Met5−.
A biochip for quantifying homocysteine, the biochip comprising Escherichia coli MetB− and Escherichia coli Met5− immobilized on a planar substrate.
Dependencies are inferred from the claim text.