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AssigneeKoninklijke Philips Electronics N.V.
InventorsGwenola Sabatte, Menno Willem Jose Prins, Toon Hendrik Evers, Wilhelmina Maria Hardeman, Joukje Garrelina Orsel
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The present invention is related to a method for detection of a biological target in an affinity assay, the method comprising the steps of providing a biological sample volume containing the biological target, adding a first capturing moiety to the biological sample volume comprising the biological target, wherein the first capturing moiety is adhered to a particle, concentration of the captured biological target into an elution volume that is smaller than the biological sample volume in step a), cleavage of the first capturing moiety or the biological target from the particle and direct or indirect detection and/or quantification of the biological target in a sandwich or competitive affinity assay format, wherein the biological target is associated with at least one capturing moiety, preferably at least two capturing moieties.
A method for the detection of a biological target in an affinity assay, the method comprising the steps of
(canceled)
A detection arrangement for a biological target comprising a biological target, a first, a second and a third capturing moiety, wherein a reagent is associated with the second and/or the third capturing moiety and the biological target is bound directly to all three capturing moieties thereby enabling detection.
Dependencies are inferred from the claim text.