US 2013/0095489 A1 · PROCESS FOR DETECTION OF MULTIDRUG RESISTANT TUBERCULOSIS USING REAL-TIME PCR AND HIGH RESOLUTION MELT ANALYSIS

Abstract

Compositions and process are provided for the rapid and specific detection of drug resistant forms of Mycobacterium tuberculosis based on real time PCR and high resolution melt analysis. The compositions and processes are useful for the detection of mutations within the Rifampicin Resistance Determinant Region (RRDR) of rpoB for the detection of rifampicin (RIF) and within specific regions of katG and the inhA promoter for the detection of isoniazid (INH) resistance. The invention also is capable of rapidly discriminating Mycobacterium tuberculosis complex (MTBC) strains from Nontuberculous Mycobacteria (NTM) strains.

Claims22

Dependent claims

A process for detecting drug resistance in a Mycobacterium tuberculosis strain, comprising:

exposing a DNA sample to a primer pair comprising a forward primer and a reverse primer under conditions conducive to a polymerase chain reaction to yield an amplicon, wherein said primer pair is specific to the region of rpoB, katG or inhA promoter of the Mycobacterium tuberculosis strain;
and
detecting said amplicon indicative of the Mycobacterium tuberculosis strain.

Dependencies are inferred from the claim text.

Process for detection of multidrug resistant tuberculosis using real-time pcr and high resolution melt analysis

Life of this patent

Process for detection of multidrug resistant tuberculosis using real-time pcr and high resolution melt analysis

Life of this patent

Abstract

Claims22