HtSNPs FOR DETERMINING A GENOTYPE OF CYTOCHROME P450 1A2, 2A6 AND 2D6, PXR AND UDP-GLUCURONOSYLTRANSFERASE 1A GENE AND MULTIPLEX GENOTYPING METHODS USING THEREOF

A method of selecting htSNPs in a human CYP2A6 gene, the method comprising:

• (a) collecting a biological sample from subjects;
• (b) extracting nucleic acid from the sample collected at operation (a);
• (c) performing PCR with a primer which amplifies a human CYP2A6 gene or a fragment thereof by using the nucleic acid extracted at operation (b) as a template;
• (d) determining a presence of variants in genetic sequences of a PCR product obtained at operation (c);
• (e) determining a haplotype from the genetic sequences of the PCR product that is determined to have the variant at operation (d);
• and
• (f) sequencing the haplotype determined at operation
• (e) with SNPtagger software and selecting htSNPs.