Method for determination of target cells or tissue for extraction of biomolecules from fixed biological samples
AssigneeQiagen GmbH
InventorDaniel Groelz
Life of this patent
Estimating the term…
Abstract
The present invention relates to a method for determination of target cells or tissue for isolating or extracting biomolecules from fixed biological samples, the preparation of a sample in a method for extracting, isolating and/or purifying biomolecules from a fixed biological sample as well as to a kit for visualizing target cells or tissue in a fixed biological sample for extracting, isolating and/or purifying biomolecules from said target cells or tissue.
Claims29
-14. (canceled)
A method for determining target cells or tissue for isolation, extraction or purification of biomolecules from a fixed biological sample, comprising:
- (1) mounting at least one part of the fixed biological sample on at least one support,
- (2) optionally treating the at least one part of the sample with a sample treatment agent, wherein said treatment can be carried out at any stage of the procedure before step (6),
- (3) staining the at least one part of the fixed biological sample mounted on the at least one support,
- (4) considering the at least one part stained in step (3) to determine target cells or tissue comprising biomolecules of interest,
- (5) separating target cells or tissue comprising biomolecules of interest from the remainder of the fixed sample mounted on the at least one support, and
- (6) isolating the biomolecules from the separated target cells or tissue.
The method according to claim 14, wherein the fixed biological sample is a wax embedded sample, and wherein the sample treatment agent is a de-waxing agent.
A method for staining a fixed biological sample, comprising:
- (a) contacting a fixed biological sample for not more than 60 seconds with a first tissue staining agent,
- (b) rinsing the sample with water, and
- (c) thereafter optionally contacting the sample for less than 10 seconds with a second tissue staining agent.
Dependencies are inferred from the claim text.