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AssigneesHeineken Supply Chain B.V., Carlsberg Breweries A/S
InventorsSøren Knudsen, Preben Rils, Birgitte Skadhauge, Lene Mølskov Bech, Ole Olsen
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Barley based beverages are produced in large quantities, employing highly energy consuming methods, for example in the malting and brewhouse facilities for kiln drying and wort boiling operations, respectively. The present invention relates to energy saving methods for preparing barley based beverages, as well as to barley plants useful in such methods. In particular, the invention describes barley plants with combined traits of null-lipoxygenase-1 (null-LOX-1), null-lipoxygenase-2 (null-LOX-2) and null-S-adenosylmethionine:methionine S-methyltransferase in one plant, which is particularly useful for energy saving methods to prepare barley based beverages, such as beer.
A method for preparing a cereal based beverage with low levels of one or more off-flavours and/or precursors thereof, wherein the method involves reduced energy input, the method comprising the steps of:
The method according to anyone of the preceding claims, wherein said off-flavours are dimethyl sulphide (DMS) and trans-2-nonenal (T2N).
The method according to anyone of the preceding claims, wherein said precursors of off-flavours are S-methyl-methionine (SMM) and T2N potential.
The method according to anyone of the preceding claims, wherein all of said provided barley is malted.
The method according to anyone of the preceding claims, wherein said barley is malted using a method comprising the steps of
The method according to anyone of the preceding claims, wherein said barley and/or said malted barley is milled prior to said mashing.
The method according to anyone of the preceding claims, wherein the wort composition has been produced by a method involving a mashing step using a mashing in temperature not exceeding 69° C.
The method according to anyone of the preceding claims, wherein the temperature during mashing at no time exceeds 80° C., preferably 78° C.
The method according to anyone of the preceding claims, wherein additional adjunct(s) are added in step (iii), and said additional adjuncts are selected from the group consisting of maize, rice and unmalted cereal other than barley.
The method according to anyone of the preceding claims, wherein additional ingredient(s) are added in step (iv) and said additional ingredient is hops or an extract thereof.
The method according to anyone of the preceding claims, wherein said wort is heated to a temperature of at the most 99.8° C., such as at the most 99.5° C., for example at the most 99° C.
The method according to anyone of the preceding claims, wherein step (iv) concerning heating said wort is performed with the wort in an essentially closed container, preferably with the wort in a closed container.
The method according to anyone of the preceding claims, wherein said wort is heated to a temperature above 80° C. for at the most 30 min, preferably at the most 20 min.
The method according to anyone of the preceding claims, wherein at the most 3%, such as at the most 2%, for example at the most 1%, such as at the most 0.5%, for example at the most 0.1%, such as at the most 0.01% of the wort volume is evaporated.
The method according to anyone of the preceding claims, wherein step v) comprises cooling said heated wort.
The method according to anyone of the preceding claims, wherein step v) comprises fermentation of the wort.
The method according to anyone of the preceding claims, wherein the method does not involve heating to a temperature of more than 99° C. at any step.
The method according to anyone of the preceding claims, wherein the method does not involve heating to a temperature above 80° C. for more than 30 min, preferably for more than 20 min.
A barley plant, or a part thereof, wherein said barley plant comprises:
The barley plant, or a part thereof, according to any one of claims to 27 to 29, wherein the gene encoding LOX-1 of said plant comprises a premature stop codon situated at the most 705, preferably at the most 665 codons downstream of the start codon.
Dependencies are inferred from the claim text.