Bone morphogenic protein binding peptide
AssigneesDepartment of Veterans Affairs, The Regents of the University of California
InventorsSamuel S. Murray, Elsa J. Brochmann-Murray, Jeffrey Wang, Keyvan Behnam
Life of this patent
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Abstract
A cyclized peptide designated BMP Binding Peptide (BBP) is a synthetic peptide that avidly binds rhBMP-2, as do endogenous forms of BBP, and sequence conservation between species results in a variety of useful BBP compositions. BBP increases the over-all osteogenic activity of rhBMP-2, increases the rate at which rhBMP-2 induces bone formation, and BBP induces calcification alone. Compositions and substrates including BBP, and methods of using BBP are useful in therapeutic, diagnostic and clinical applications requiring calcification and osteogenesis.
Claims44
1-25. (canceled)
An article of manufacture comprising an implant, wherein said implant has a surface, and wherein said surface includes a peptide having the amino acid sequence of SEQ ID No. 13, or a fragment thereof, wherein said peptide or fragment increases the degree or rate of osteogenesis or calcification in vertebrates.
An article of manufacture comprising a solid support, wherein said solid support includes a peptide having the amino acid sequence of SEQ ID No. 13, or a fragment thereof, wherein said peptide or fragment increases the degree or rate of osteogenesis or calcification in vertebrates.
A method of enhancing the rate or degree of osteogenesis or calcification in vertebrate tissue comprising:
- (a) treating mesenchymal stem cells with one or more of TGF-beta, BMP-2, BMP-4, BMP-7 or demineralized bone matrix;
- (b) treating mesenchymal stems cells with a peptide having the amino acid sequence of SEQ ID No. 13, or a fragment thereof, wherein said peptide or fragment increases the degree or rate of osteogenesis or calcification;
- and
- (c) administering the mesenchymal stem cells to the tissue.
A method of inducing osteogenesis or calcification in a vertebrate comprising:
- (a) treating mesenchymal stem cells with one or more of TGF-beta, BMP-2, BMP-4, BMP-7 or demineralized bone matrix;
- (b) treating mesenchymal stems cells with a peptide having the amino acid sequence of SEQ ID No. 13, or a fragment thereof, wherein said peptide or fragment increases the degree or rate of osteogenesis or calcification;
- and
- (c) administering the mesenchymal stem cells to the vertebrate.
A method of enhancing the degree or rate of osteogenesis or calcification in vertebrate tissue comprising applying to the tissue an implant, wherein said implant has a surface, and wherein said surface includes a peptide having the amino acid sequence of SEQ ID No. 13, or a fragment thereof, wherein said peptide or fragment increases the degree or rate of osteogenesis or calcification in vertebrates.
A method of inducing osteogenesis or calcification in vertebrate tissue comprising administering to the tissue chondrogenic or osteogenic precursor cells and a peptide having the amino acid sequence of SEQ ID No. 13, or a fragment thereof, wherein said peptide or fragment increases the degree or rate of osteogenesis or calcification.
A method of inducing osteogenesis or calcification in vertebrate tissue comprising administering to the tissue mesenchymal stem cells and a peptide having the amino acid sequence of SEQ ID No. 13, or a fragment thereof, wherein said peptide or fragment increases the degree or rate of osteogenesis or calcification.
A method of differentiating mesenchymal stem cells comprising treating mesenchymal stems cells with a peptide having the amino acid sequence of SEQ ID No. 13, or a fragment thereof, wherein said peptide or fragment increases the degree or rate of osteogenesis or calcification.
Dependencies are inferred from the claim text.