Pharmaceutical compositions comprising a polypeptide comprising at least one cxxc motif and heterologous antigens and uses thereof
AssigneesInserm (Institut National De La Sante Et De La Recherche Medicale), Centre National de la Recherche Scientifique (CNRS), Universidad Catolica de Cordoba (UCC), Assistance Publique-Hopitaux de Paris, Consejo Nacional De Investigaciones Cientificas Y Tecnicas (Conicet), Universite Pierre Et Marie Curie (Paris 6)
InventorsDavid Klatzmann, Eliane Piaggio, Hugo Daniel Luján
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Abstract
The invention relates to pharmaceutical compositions using a polypeptide comprising at least one CXXC motif, such as Giardia parasite's variable surface proteins (VSP) or a fragment thereof to raise by oral or mucosal vaccination an immune response against a heterologous selected antigen, such as tumor antigen, microbial antigen or other antigen.
Claims21
A vaccine against an antigen comprising:
- an antigen bound to a polypeptide,
- wherein the polypeptide:
- is heterologous to the antigen
- comprises at least two CXXC motifs, wherein C represents a cysteine residue and X any aminoacid residue, and
- is able to attach to epithelial cells of the gut.
4-5. (canceled)
7-26. (canceled)
The vaccine according to claim 8, wherein the microorganism is selected in the group consisting of Giardia, Tetrahymena, Paramecium and Entamoeba species.
The vaccine according to claim 8, wherein the polypeptide is the extracellular domain of VSP or a fragment thereof.
The vaccine according to claim 14, wherein the antigen is contained inside or on the surface of the VLP.
The vaccine according to claim 12, wherein the vector particle is a nanoparticule displaying at its surface the polypeptide and the heterologous antigen.
A method of immunization of a subject against an antigen, wherein said method comprises administering to said subject said antigen bound to a polypeptide,
- wherein the polypeptide:
- is heterologous to said antigen
- comprises at least two CXXC motifs, wherein C represents a cysteine residue and X any aminoacid residue, and
- is able to attach to epithelial cells of the gut.
The method of claim 18, wherein said antigen bound to a polypeptide is administered by oral route.
Dependencies are inferred from the claim text.