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AssigneeUniversity of Maryland, College Park
InventorsSilvia Muro-Galindo, Daniel Serrano
Estimating the term…
Methods for controlling and regulating engulfment, uptake and/or transcellular transport at a stage following initial engagement of an agent to the endothelium are provided, based on the identification of CAM-mediated endocytosis and the sphingomyelin/ceramide pathway as active steps in transcellular TEM. Administration of regulators relating to the identified pathways, such as NHE1, sphingomyelinases, acid sphingomyelinase and ceramide, permit control and regulation of uptake and transcellular transport. Control and regulation of uptake and/or transcellular transport is applicable in strategies to modulate inflammation, provide controlled and/or targeted delivery of agents, control pathogenic invasion, recover action of an inhibited CAM-mediated uptake or transendothelial pathway, or provide uptake or transendothelial transport by targeting cell surface markers other than ICAM-1.
A method of regulating formation of engulfment or docking structures, uptake or transcellular transport of an agent, the method comprising administration of a regulator of CAM-mediated endocytosis or the sphingomyelin/ceramide pathway, wherein such administration is effective to regulate engulfment, uptake or transcellular transport of the agent.
A method of recovery of an inhibited pathway, the method comprising administration of a regulator of CAM-mediated endocytosis or the sphingomyelin/ceramide pathway, wherein such administration is effective to regulate engulfment, uptake or transcellular transport of the agent and wherein the inhibition is inhibition of engulfment, uptake or transcellular transport within a pathway selected from the group consisting of CAM-mediated endocytosis, phagocytosis, macropinocytosis, clathrin-mediated endocytosis and caveolar-mediated endocytosis.
A method of potentiating engulfment, uptake or transcellular migration of an agent, wherein the agent is complexed to a carrier targeted to a non-ICAM cell surface molecule or receptor, the method comprising administration of a regulator of CAM-mediated endocytosis or the sphingomyelin/ceramide pathway, wherein such administration is effective to induce uptake of the agent and potentiation of transcellular transport of the agent.
A method of modulating inflammation, comprising administration of a regulator of CAM-mediated endocytosis or the sphingomyelin/ceramide pathway, which is effective to regulate engulfment, uptake or transcellular transport of an agent of said inflammation.
Dependencies are inferred from the claim text.